Microarray based gene expression analysis of Sus Scrofa duodenum exposed to zearalenone: significance to human health
| Autor: | Daniela Eliza Marin, Roxana Cojocneanu-Petric, Alexandru Mihail Gras, Cornelia Braicu, Diana Gulei, Ionelia Taranu, Ioana Berindan-Neagoe, Ancuta Jurj |
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| Jazyk: | angličtina |
| Předmět: |
0301 basic medicine
Microarray Duodenum Sus scrofa Context (language use) Biology medicine.disease_cause Proteomics 03 medical and health sciences Gene expression medicine Genetics Animals Humans Gene Regulatory Networks Estrogens Non-Steroidal Extrapolation to human Gene Gene Expression Profiling Reproducibility of Results food and beverages Molecular biology Disease Models Animal 030104 developmental biology Gene Expression Regulation Health Gene chip analysis Cytokines Zearalenone DNA microarray Transcriptome Carcinogenesis Research Article Biotechnology |
| Zdroj: | BMC Genomics |
| ISSN: | 1471-2164 |
| DOI: | 10.1186/s12864-016-2984-8 |
| Popis: | Background Zearalenone (ZEA) is a secondary metabolite produced by Fusarium species. ZEA was proved to exert a wide range of unwanted side effects, but its mechanism of action, particularly at duodenum levels, remains unclear. In our study based on the microarray technology we assessed the alteration of gene expression pattern Sus scrofa duodenum which has been previously exposed to ZEA. Gene expression data was validated by qRT-PCR and ELISA. The gene expression data were further extrapolated the results to their human orthologues and analyzed the data in the context of human health using IPA (Ingenuity Pathways Analysis). Results Using Agilent microarray technology, we found that gene expression pattern was significantly affected by ZEA exposure, considering a 2-fold expression difference as a cut-off level and a p-value |
| Databáze: | OpenAIRE |
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