Bronchoalveolar lavage (BAL) cells in idiopathic pulmonary fibrosis express a complex pro-inflammatory, pro-repair, angiogenic activation pattern, likely associated with macrophage iron accumulation
| Autor: | C. Magnus Sköld, Cesare Saltini, Marco Pallante, Carmen M. Swaisgood, Mark L. Brantly, Lazarus K. Mramba, Massimo Amicosante, Ivan Arisi, Ermanno Puxeddu, Jungnam Lee |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male 0301 basic medicine Iron medicine.medical_treatment lcsh:Medicine CCL4 03 medical and health sciences Idiopathic pulmonary fibrosis 0302 clinical medicine Pulmonary fibrosis medicine Humans CXCL10 lcsh:Science Aged Inflammation Multidisciplinary Neovascularization Pathologic medicine.diagnostic_test Sequence Analysis RNA business.industry Gene Expression Profiling Macrophages lcsh:R Correction Middle Aged respiratory system medicine.disease Idiopathic Pulmonary Fibrosis 3. Good health CXCL2 Phenotype 030104 developmental biology Bronchoalveolar lavage Cytokine 030228 respiratory system Alveolar macrophage Cancer research Female lcsh:Q Chemokines Reactive Oxygen Species business Bronchoalveolar Lavage Fluid |
| Zdroj: | PLoS ONE, Vol 13, Iss 4, p e0194803 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease of unknown cause characterized by alveolar epithelial damage, patchy interstitial fibrosis and diffuse microvascular abnormalities. In IPF, alveolar clustering of iron-laden alveolar macrophages-a common sign of microhemorrhage, has been associated with vascular abnormalities and worsening of pulmonary hypertension. As iron-dependent ROS generation has been shown to induce unrestrained macrophage activation in disease models of vascular damage, we explored alveolar macrophage activation phenotype in IPF patients (n = 16) and healthy controls (CTR, n = 7) by RNA sequencing of bronchoalveolar lavage (BAL) cells. The frequencies of macrophages in BAL cells were 86+4% and 83.4+8% in IPF and CTR groups, respectively (p-value = 0.41). In IPF patients, BAL cells showed increased iron-dependent ROS generation (p-value |
| Databáze: | OpenAIRE |
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