Improved rat spinal cord injury model using spinal cord compression by percutaneous method
| Autor: | A-Jin Lee, Soo-Han Hwang, Hoon Han, Dai-Jung Chung, Sun Hee Do, Hyo Jin Chung, Hyun Jung Suh, Wojong Yang, Chi-Bong Choi, Hwa-Seok Chang, Jae-Hoon Lee, Dae-Hyun Kim, Hwi-Yool Kim, Wook-Hun Chung |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Percutaneous Rats Sprague-Dawley Spinal cord compression laminectomy-free medicine Paralysis Animals magnetic resonance imaging rat Spinal cord injury General Veterinary business.industry Balloon catheter medicine.disease Spinal cord Epidural space Surgery Rats percutaneous spinal cord injury balloon compression Disease Models Animal medicine.anatomical_structure Anesthesia Original Article Balloon Embolectomy medicine.symptom business Spinal Cord Compression Lumbosacral joint |
| Zdroj: | Journal of Veterinary Science |
| ISSN: | 1976-555X |
| Popis: | Here, percutaneous spinal cord injury (SCI) methods using a balloon catheter in adult rats are described. A balloon catheter was inserted into the epidural space through the lumbosacral junction and then inflated between T9-T10 for 10 min under fluoroscopic guidance. Animals were divided into three groups with respect to inflation volume: 20 μL (n = 18), 50 μL (n = 18) and control (Fogarty catheter inserted but not inflated; n = 10). Neurological assessments were then made based on BBB score, magnetic resonance imaging and histopathology. Both inflation volumes produced complete paralysis. Gradual recovery of motor function occurred when 20 μL was used, but not after 50 μL was applied. In the 50 μL group, all gray and white matter was lost from the center of the lesion. In addition, supramaximal damage was noted, which likely prevented spontaneous recovery. This percutaneous spinal cord compression injury model is simple, rapid with high reproducibility and the potential to serve as a useful tool for investigation of pathophysiology and possible protective treatments of SCI in vivo. |
| Databáze: | OpenAIRE |
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