1H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations
| Autor: | Peter Vermathen, Nico Ruprecht, Hendrik von Tengg-Kobligk, Martin Hungerbühler, Martina Vermathen |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Taurine
Cell Pharmaceutical Science cisplatin Organic chemistry 610 Medicine & health Drug resistance NSCLC Article Analytical Chemistry chemistry.chemical_compound Metabolomics QD241-441 Drug Discovery 540 Chemistry medicine Physical and Theoretical Chemistry Lung cancer non-small cell lung cancer Cisplatin Glutathione medicine.disease cisPt resistance metabolomics medicine.anatomical_structure chemistry Chemistry (miscellaneous) Cell culture Cancer research HR-MAS NMR Molecular Medicine 570 Life sciences biology medicine.drug |
| Zdroj: | Vermathen, Martina; von Tengg-Kobligk, Hendrik; Hungerbühler, Martin Nils; Vermathen, Peter; Ruprecht, Nico (2021). 1H HR-MAS NMR Based Metabolic Profiling of Lung Cancer Cells with Induced and De-Induced Cisplatin Resistance to Reveal Metabolic Resistance Adaptations. Molecules, 26(22), p. 6766. Molecular Diversity Preservation International MDPI 10.3390/molecules26226766 Molecules, Vol 26, Iss 6766, p 6766 (2021) Molecules Volume 26 Issue 22 |
| DOI: | 10.3390/molecules26226766 |
| Popis: | Cisplatin (cisPt) is an important drug that is used against various cancers, including advanced lung cancer. However, drug resistance is still a major ongoing problem and its investigation is of paramount interest. Here, a high-resolution magic angle spinning (HR-MAS) NMR study is presented deciphering the metabolic profile of non-small cell lung cancer (NSCLC) cells and metabolic adaptations at different levels of induced cisPt-resistance, as well as in their de-induced counterparts (cells cultivated in absence of cisPt). In total, fifty-three metabolites were identified and quantified in the 1H-HR-MAS NMR cell spectra. Metabolic adaptations to cisPt-resistance were detected, which correlated with the degree of resistance. Importantly, de-induced cell lines demonstrated similar metabolic adaptations as the corresponding cisPt-resistant cell lines. Metabolites predominantly changed in cisPt resistant cells and their de-induced counterparts include glutathione and taurine. Characteristic metabolic patterns for cisPt resistance may become relevant as biomarkers in cancer medicine. |
| Databáze: | OpenAIRE |
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