Characterization, optimization, and scale-up of cellulases production by trichoderma reesei cbs 836.91 in solid-state fermentation using agro-industrial products
| Autor: | Martín Blasco, Edgardo Albertó, Gastón Ezequiel Ortiz, Sebastian Fernando Cavalitto, Marcelo Fernández-Lahore, María E. Guitart |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Dietary Fiber
Trichoderma reesei Drum bioreactor Bioengineering Cellulase INGENIERÍAS Y TECNOLOGÍAS Biotecnología Industrial Fungal Proteins Hydrolysis Solid-state fermentation Cellulases Food science Trichoderma Cellulases production Fungal protein biology Bran Chemistry business.industry General Medicine biology.organism_classification Bioprocesamiento Tecnológico Biocatálisis Fermentación Biotechnology biology.protein Industrial and production engineering business Design of experiments |
| DOI: | 10.1007/s00449-015-1451-2 |
| Popis: | The application of cellulases in saccharification processes is restricted by its production cost. Consequently, new fungal strains able to elaborate higher cellulases titers and with special activity profiles are required to make the process economical. The aim of this investigation was to find a promising wild-type Trichoderma strain for cellulases production. The Trichoderma reesei strain 938 (CBS 836.91) was selected among twenty strains on the basis of cellulase-agar-plate screening. Evaluation of the selected strain on six solid substrates indicated the highest activities to be obtained from wheat bran. Statistical analyses of the experimental design indicated a significant effect of pH and moisture on the generation of endoglucanase (EGA) and filter-paper (FPA) activity. Furthermore, a central-composite design-based optimization revealed that pH values between 6.4 and 6.6 and moisture from 74 to 94 % were optimal for cellulases production. Under these conditions, 8?10 IU gds-1 of FPA and 15.6?17.8 IU gds-1 of EGA were obtained. In addition, cultivation in a rotating-drum reactor under optimal conditions gave 8.2 IU gds-1 FPA and 13.5 IU gds-1 EGA. Biochemical characterization of T. reesei 938 cellulases indicated a substantially higher resistance to 4 mM Fe?2 and a slightly greater tolerance to alkaline pH in comparison to Celluclast. These results suggest that T. reesei 938 could be a promising candidate for improved cellulases production through direct-evolution strategies. Fil: Ortiz, Gastón Ezequiel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania Fil: Guitart, María Eugenia. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Química y Físico-Química Biológicas "Prof. Alejandro C. Paladini". Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Química y Físico-Química Biológicas; Argentina Fil: Cavalitto, Sebastian Fernando. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - la Plata. Centro de Investigación y Desarrollo En Fermentaciones Industriales. Universidad Nacional de la Plata. Facultad de Cs.exactas. Centro de Investigación y Desarrollo En Fermentaciones Industriales; Argentina Fil: Alberto, Edgardo Omar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús). Universidad Nacional de San Martín. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas "Dr. Raúl Alfonsín" (sede Chascomús); Argentina Fil: Fernandez Lahore, Marcelo. Universitat Bremen. School Of Enigineerring And Science Jacobs; Alemania Fil: Blasco, Martín. Instituto Nacional de Tecnología Industrial. Centro de Investigación y Desarrollo en Biotecnología Industrial; Argentina |
| Databáze: | OpenAIRE |
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