Measurement of menadione in urine by HPLC
| Autor: | Ala Al Rajabi, John W. Suttie, Sang Woon Choi, James W. Peterson, Susan Barakat, Sarah L. Booth |
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| Rok vydání: | 2010 |
| Předmět: |
Calibration curve
Metabolite Clinical Biochemistry Urine Sensitivity and Specificity Biochemistry High-performance liquid chromatography Article Analytical Chemistry chemistry.chemical_compound Drug Stability Menadione Humans Sample preparation Chromatography High Pressure Liquid Clinical Trials as Topic Chromatography Chemistry Methanol Reproducibility of Results Vitamin K 3 Water Vitamin K 2 Vitamin K 1 Cell Biology General Medicine Menadiol Linear Models Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography B. 878:2457-2460 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2010.07.016 |
| Popis: | Menadione is a metabolite of vitamin K that is excreted in urine. A high performance liquid chromatography (HPLC) method using a C30 column, post-column zinc reduction and fluorescence detection was developed to measure urinary menadione. The mobile phase was composed of 95% methanol with 0.55% aqueous solution and 5% DI H2O. Menaquinone-2 (MK-2) was used as an internal standard. The standard calibration curve was linear with a correlation coefficient (R2) of 0.999 for both menadione and MK-2. The lower limit of quantification (LLOQ) was 0.3 pmole menadione/mL urine. Sample preparation involved hydrolysis of menadiol conjugates and oxidizing the released menadiol to menadione. Using this method, urinary menadione was shown to increase in response to 3 years of phylloquinone supplementation. This HPLC method is a sensitive and reproducible way to detect menadione in urine. Research support: USDA ARS Cooperative Agreement 58-1950-7-707. |
| Databáze: | OpenAIRE |
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