Mechanical and biocompatible characterization of a cross-linked collagen-hyaluronic acid wound dressing
Autor: | James D. Talton, Chandra Nataraj, Ronald R. Cobb, James F. Kirk, José Luis Millán, Dhyana Sankar, Sonoko Narisawa, Joseph D. Reddy, Isaac Finger, Gregg Ritter |
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Rok vydání: | 2013 |
Předmět: |
Chronic wound
Scaffold Materials science Cell Survival Biomedical Engineering Medicine (miscellaneous) Biocompatible Materials Matrix (biology) Cell Line histology Biomaterials Glycosaminoglycan Mice chemistry.chemical_compound wound repair Report Tensile Strength hyaluronic acid Materials Testing Hyaluronic acid medicine Animals Composite material Fibroblast Carbodiimide General Medicine Fibroblasts Cross-Linking Reagents medicine.anatomical_structure collagen sponge chemistry Biophysics Collagen Stress Mechanical Glutaraldehyde medicine.symptom cross-linking Bandages Hydrocolloid |
Zdroj: | Biomatter |
ISSN: | 2159-2535 |
Popis: | Collagen scaffolds have been widely employed as a dermal equivalent to induce fibroblast infiltrations and dermal regeneration in the treatment of chronic wounds and diabetic foot ulcers. Cross-linking methods have been developed to address the disadvantages of the rapid degradation associated with collagen-based scaffolds. To eliminate the potential drawbacks associated with glutaraldehyde cross-linking, methods using a water soluble carbodiimide have been developed. In the present study, the glycosaminoglycan (GAG) hyaluronic acid (HA), was covalently attached to an equine tendon derived collagen scaffold using 1-ethyl-3-(3-dimethyl aminopropyl) carbodiimide (EDC) to create ntSPONGE™. The HA was shown to be homogeneously distributed throughout the collagen matrix. In vitro analyses of the scaffold indicated that the cross-linking enhanced the biological stability by decreasing the enzymatic degradation and increasing the thermal denaturation temperature. The material was shown to support the attachment and proliferation of mouse L929 fibroblast cells. In addition, the cross-linking decreased the resorption rate of the collagen as measured in an intramuscular implant model in rabbits. The material was also shown to be biocompatible in a variety of in vitro and in vivo assays. These results indicate that this cross-linked collagen-HA scaffold, ntSPONGE™, has the potential for use in chronic wound healing. |
Databáze: | OpenAIRE |
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