Isolation and Optimization of Culture Conditions of Thraustochytrium kinnei for Biomass Production, Nanoparticle Synthesis, Antioxidant and Antimicrobial Activities
| Autor: | Kandasamy Kathiresan, Venugopal Gomathi, Laurent Dufossé, Kaliyamoorthy Kalidasan, Nabikhan Asmathunisha |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
thraustochytrids Antioxidant DPPH medicine.medical_treatment Naval architecture. Shipbuilding. Marine engineering Nanoparticle VM1-989 Ocean Engineering GC1-1581 Oceanography 01 natural sciences Silver nanoparticle antimicrobials 03 medical and health sciences chemistry.chemical_compound 010608 biotechnology medicine Petroleum ether Food science 030304 developmental biology Water Science and Technology Civil and Structural Engineering 0303 health sciences Strain (chemistry) mangroves Antimicrobial DHA antioxidants chemistry Colloidal gold nanoparticles |
| Zdroj: | Journal of Marine Science and Engineering, Vol 9, Iss 678, p 678 (2021) Journal of Marine Science and Engineering Volume 9 Issue 6 |
| ISSN: | 2077-1312 |
| Popis: | This work deals with the identification of a predominant thraustochytrid strain, the optimization of culture conditions, the synthesis of nanoparticles, and the evaluation of antioxidant and antimicrobial activities in biomass extracts and nanoparticles. Thraustochytrium kinnei was identified as a predominant strain from decomposing mangrove leaves, and its culture conditions were optimized for maximum biomass production of 13.53 g·L−1, with total lipids of 41.33% and DHA of 39.16% of total fatty acids. Furthermore, the strain was shown to synthesize gold and silver nanoparticles in the size ranges of 10–85 nm and 5–90 nm, respectively. Silver nanoparticles exhibited higher total antioxidant and DPPH activities than gold nanoparticles and methanol extract of the strain. The silver nanoparticles showed higher antimicrobial activity than gold nanoparticles and petroleum ether extract of the strain. Thus, Thraustochytrium kinnei is proven to be promising for synthesis of silver nanoparticles with high antioxidant and antimicrobial activity. |
| Databáze: | OpenAIRE |
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