Growth factors in multiple myeloma: a comprehensive analysis of their expression in tumor cells and bone marrow environment using Affymetrix microarrays
| Autor: | Bernard Klein, Tobias Meißner, Jérôme Moreaux, Hartmut Goldschmidt, Dirk Hose, Karène Mahtouk, Thierry Rème, Jean François Rossi, Michel Jourdan, Steven T. Pals |
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| Přispěvatelé: | Faculteit der Geneeskunde, Institut de recherche en biothérapie (IRB), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 1 (UM1)-Université de Montpellier (UM), Medizinische Klinik V, Universität Heidelberg [Heidelberg], Nationales Centrum für Tumorerkrankungen, Department of Pathology, Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA)-University of Amsterdam [Amsterdam] (UvA), This work was supported by grants from the Ligue Nationale Contre le Cancer (équipe labellisée, 2009), Paris, France, from INCA (n°R07001FN) and from BMSCNET European strep (N°E06005FF), the Hopp-Foundation, Germany, the University of Heidelberg, Germany, the National Centre for Tumor Diseases, Heidelberg, Germany, the Tumorzentrum Heidelberg/Mannheim, Germany., Pathology, AII - Amsterdam institute for Infection and Immunity, CCA -Cancer Center Amsterdam, Université Montpellier 1 (UM1)-Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM), BMC, Ed. |
| Rok vydání: | 2009 |
| Předmět: |
Cancer Research
Cellular differentiation MESH: Multiple Myeloma 0302 clinical medicine Surgical oncology MESH: Up-Regulation Multiple myeloma Oligonucleotide Array Sequence Analysis 0303 health sciences MESH: Plasma Cells MESH: Middle Aged MESH: Bone Marrow Cells Cell Differentiation MESH: Gene Expression Regulation Neoplastic MESH: Neoplasm Staging Middle Aged lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens MESH: Case-Control Studies Up-Regulation Gene Expression Regulation Neoplastic medicine.anatomical_structure Oncology 030220 oncology & carcinogenesis Intercellular Signaling Peptides and Proteins Multiple Myeloma Research Article MESH: Cell Differentiation Plasma Cells Tumor cells [SDV.CAN]Life Sciences [q-bio]/Cancer Bone Marrow Cells Biology lcsh:RC254-282 03 medical and health sciences MESH: Gene Expression Profiling [SDV.CAN] Life Sciences [q-bio]/Cancer Downregulation and upregulation MESH: Receptors Growth Factor medicine Genetics Humans Receptors Growth Factor RNA Messenger MESH: Intercellular Signaling Peptides and Proteins Affymetrix microarrays 030304 developmental biology MESH: RNA Messenger Neoplasm Staging MESH: Humans Gene Expression Profiling medicine.disease Molecular biology Gene expression profiling Case-Control Studies MESH: Oligonucleotide Array Sequence Analysis Bone marrow |
| Zdroj: | BMC Cancer BMC Cancer, 10(1). BioMed Central BMC Cancer, BioMed Central, 2010, 10 (1), pp.198. ⟨10.1186/1471-2407-10-198⟩ BMC cancer, 10(1). BioMed Central BMC Cancer, Vol 10, Iss 1, p 198 (2010) |
| ISSN: | 1471-2407 |
| DOI: | 10.1186/1471-2407-10-198⟩ |
| Popis: | Background Multiple myeloma (MM) is characterized by a strong dependence of the tumor cells on their microenvironment, which produces growth factors supporting survival and proliferation of myeloma cells (MMC). In the past few years, many myeloma growth factors (MGF) have been described in the literature. However, their relative importance and the nature of the cells producing MGF remain unidentified for many of them. Methods We have analysed the expression of 51 MGF and 36 MGF receptors (MGFR) using Affymetrix microarrays throughout normal plasma cell differentiation, in MMC and in cells from the bone marrow (BM) microenvironment (CD14, CD3, polymorphonuclear neutrophils, stromal cells and osteoclasts). Results 4/51 MGF and 9/36 MGF-receptors genes were significantly overexpressed in plasmablasts (PPC) and BM plasma cell (BMPC) compared to B cells whereas 11 MGF and 11 MGFR genes were overexpressed in BMPC compared to PPC. 3 MGF genes (AREG, NRG3, Wnt5A) and none of the receptors were significantly overexpressed in MMC versus BMPC. Furthermore, 3/51 MGF genes were overexpressed in MMC compared to the the BM microenvironment whereas 22/51 MGF genes were overexpressed in one environment subpopulation compared to MMC. Conclusions Two major messages arise from this analysis 1) The majority of MGF genes is expressed by the bone marrow environment. 2) Several MGF and their receptors are overexpressed throughout normal plasma cell differentiation. This study provides an extensive and comparative analysis of MGF expression in plasma cell differentiation and in MM and gives new insights in the understanding of intercellular communication signals in MM. |
| Databáze: | OpenAIRE |
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