Suitability of EGCG as a Means of Stabilizing a Porcine Osteochondral Xenograft
| Autor: | Paul Gloth, Steven H. Elder, Jaylyn Walker, John Clune |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
epigallocatechin gallate Materials science lcsh:Biotechnology Biomedical Engineering 02 engineering and technology Epigallocatechin gallate complex mixtures Article Biomaterials 03 medical and health sciences chemistry.chemical_compound lcsh:TP248.13-248.65 medicine crosslinking heterocyclic compounds Composite material Cytotoxicity lcsh:R5-920 Decellularization Cartilage technology industry and agriculture Egcg treatment food and beverages osteochondral xenograft 021001 nanoscience & nanotechnology In vitro biocompatibility 030104 developmental biology medicine.anatomical_structure chemistry Collagenase Biophysics Glutaraldehyde sense organs lcsh:Medicine (General) 0210 nano-technology medicine.drug |
| Zdroj: | Journal of Functional Biomaterials; Volume 8; Issue 4; Pages: 43 Journal of Functional Biomaterials Journal of Functional Biomaterials, Vol 8, Iss 4, p 43 (2017) |
| ISSN: | 2079-4983 |
| DOI: | 10.3390/jfb8040043 |
| Popis: | As a non-crosslinked osteochondral xenograft would be mechanically inferior to native cartilage and vulnerable to premature degradation, we seek a safe and effective method of xenograft stabilization. The purpose of this study was to evaluate the capacity for epigallocatechin gallate (EGCG) to stabilize a decellularized porcine osteochondral xenograft through collagen crosslinking. Our objectives were to assess the effects of EGCG on the degree of crosslinking, mechanical properties, collagenase resistance, cytotoxicity, and in vitro biocompatibility. EGCG is a green tea polyphenol that acts as a collagen crosslinker. Porcine osteochondral plugs were decellularized and then crosslinked by soaking in EGCG. The degree of crosslinking, cartilage compressive stiffness, cartilage-bone interface strength, coefficient of friction, and residual mass after collagenase exposure all increased with an increasing EGCG concentration. With the exception of the coefficient of friction, EGCG treatment could restore mechanical properties to levels equal to, or exceeding those, of native cartilage. EGCG treatment profoundly increased the enzymatic resistance, and 1% EGCG provided protection equivalent to 1% glutaraldehyde. EGCG up to 0.5 mM was essentially not cytotoxic to chondrocytes embedded in alginate, and autologous chondrocytes attached to decellularized, EGCG-fixed cartilage were all viable five days after seeding. Results demonstrate that EGCG has many beneficial effects on a decellularized osteochondral xenograft, and may be suitable for use in stabilizing such a graft prior to implantation for the repair of a defect. |
| Databáze: | OpenAIRE |
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