Characterization of meningococcal carriage isolates from Greece by whole genome sequencing: Implications for 4CMenB vaccine implementation
| Autor: | Holly B. Bratcher, Muhamed-Kheir Taha, Athanasia Xirogianni, Konstantinos Kesanopoulos, Georgina Tzanakaki, Martin C. J. Maiden, Anastasia Papandreou, Eva Hong |
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| Přispěvatelé: | National Meningitis Reference Laboratory, National School of Public Health Athens, Department of Zoology [Oxford], University of Oxford [Oxford], Infections Bactériennes Invasives, Institut Pasteur [Paris], Funding for this study was provided by GlaxoSmithKline Biologicals SA., University of Oxford, Institut Pasteur [Paris] (IP) |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Physiology Glycobiology Neisseria meningitidis Serogroup B Pathology and Laboratory Medicine MESH: Genome Bacterial medicine.disease_cause Biochemistry MESH: Meningococcal Vaccines Serology Database and Informatics Methods Antigen Encapsulation [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases Immune Physiology Medicine and Health Sciences MESH: Genetic Variation Drug Delivery System Preparation MESH: High-Throughput Nucleotide Sequencing Vaccines education.field_of_study Immune System Proteins Multidisciplinary Greece Pharmaceutics Neisseria meningitidis MESH: Meningitis Meningococcal High-Throughput Nucleotide Sequencing Antigenic Variation Bacterial Pathogens 3. Good health Infectious Diseases Medical Microbiology Medicine Female Pathogens Neisseria Sequence Analysis Research Article Infectious Disease Control Bioinformatics Science Immunology 030106 microbiology Population Sequence Databases Meningococcal Vaccines Meningitis Meningococcal Biology Research and Analysis Methods Microbiology Herd immunity 03 medical and health sciences Antigen Polysaccharides MESH: Neisseria meningitidis Serogroup B Antigenic variation medicine Humans Typing Antigens education Microbial Pathogens DNA sequence analysis MESH: Humans Bacteria Pharmaceutical Processing Technology Organisms Genetic Variation Biology and Life Sciences Proteins Virology MESH: Male Biological Databases Carriage MESH: Greece Conjugate Vaccines MESH: Genome-Wide Association Study [SDV.IMM.VAC]Life Sciences [q-bio]/Immunology/Vaccinology MESH: Female Genome Bacterial Neisseria Meningitidis Genome-Wide Association Study |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2018, 13 (12), pp.e0209919. ⟨10.1371/journal.pone.0209919⟩ PLoS ONE, 2018, 13 (12), pp.e0209919. ⟨10.1371/journal.pone.0209919⟩ PLoS ONE, Vol 13, Iss 12, p e0209919 (2018) |
| ISSN: | 1932-6203 |
| Popis: | International audience; Herd protection, resulting from the interruption of transmission and asymptomatic carriage, is an important element of the effectiveness of vaccines against the meningococcus. Whilst this has been well established for conjugate polysaccharide vaccines directed against the meningococcal capsule, two uncertainties surround the potential herd protection provided by the novel protein-based vaccines that are used in place of serogroup B (MenB) polysaccharide vaccines (i) the strain coverage of such vaccines against carried meningococci, which are highly diverse; and (ii) the generation of a protective immune response in the mucosa. These considerations are essential for realistic estimates of cost-effectiveness of new MenB vaccines. Here the first of these questions is addressed by the whole genome sequence (WGS) analysis of meningococci isolated from healthy military recruits and university students in Greece. The study included a total of 71 MenB isolates obtained from 1420 oropharyngeal single swab samples collected from military recruits and university students on voluntary basis, aged 18-26 years. In addition to WGS analysis to identify genetic lineage and vaccine antigen genes, including the Bexsero Antigen Sequence Type (BAST), the isolates were examined with the serological Meningococcal antigen Typing System (MATS) assay. Comparison of these data demonstrated that the carried meningococcal population was highly diverse with 38% of the carriage isolates showed expression of antigens matching those included in the 4CMenB vaccine. Our data may suggest a limited potential herd immunity to be expected and be driven by an impact on a subset of carriage isolates. |
| Databáze: | OpenAIRE |
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