A transcription activator-like effector (TALE) induction system mediated by proteolysis
| Autor: | Charles B Johnson, Matthew F. Copeland, Brian F. Pfleger, Mark C. Politz, Andrew L. Markley |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Virulence Factors Repressor TALEs Biology Article 03 medical and health sciences Synthetic biology LacI Endopeptidases TEV protease Escherichia coli Promoter Regions Genetic Molecular Biology Gene Transcription factor Psychological repression Genetics Regulation of gene expression Effector Cell Biology Cell biology Repressor Proteins 030104 developmental biology Gene Expression Regulation Proteolysis induction systems Synthetic Biology trans-regulators Genetic Engineering Plasmids Transcription Factors |
| Zdroj: | Nature chemical biology |
| ISSN: | 1552-4469 |
| Popis: | Simple and predictable trans-acting regulatory tools are needed in the fields of synthetic biology and metabolic engineering to build complex genetic circuits and optimize the levels of native and heterologous gene products. Transcription activator-like effectors (TALEs) are bacterial virulence factors that have recently gained traction in biotechnology applications due to their customizable DNA binding specificity. In this work we expand the versatility of these transcription factors to create an inducible TALE system by inserting tobacco-etch virus (TEV) protease recognition sites into the TALE backbone. The resulting engineered TALEs maintain transcriptional repression of their target genes in Escherichia coli, but are degraded following the induction of the TEV protease, thereby promoting expression of the previously repressed target gene of interest. This TALE-TEV technology enables both repression and induction of plasmid or chromosomal target genes in a manner analogous to traditional repressor proteins but with the added flexibility of being operator agnostic. |
| Databáze: | OpenAIRE |
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