Xolloid-related: a novel BMP1/Tolloid-related metalloprotease is expressed during early Xenopus development
| Autor: | Leslie Dale, Shelley A Goodman, Wayne Evans |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
medicine.medical_specialty
Embryology DNA Complementary Time Factors animal structures Transcription Genetic Tolloid-Like Metalloproteinases Xenopus Molecular Sequence Data Hindbrain Bone Morphogenetic Protein 4 Xenopus Proteins Biology Transfection Pronephric duct Fungal Proteins Internal medicine medicine Animals Amino Acid Sequence RNA Messenger Noggin Phylogeny Glycoproteins Base Sequence Endoderm Gene Expression Regulation Developmental Metalloendopeptidases Proteins biology.organism_classification Cell biology Rhombencephalon medicine.anatomical_structure Endocrinology Spinal Cord Neurula Bone Morphogenetic Proteins embryonic structures Intercellular Signaling Peptides and Proteins RNA Otic vesicle Mitogen-Activated Protein Kinases Chordin Signal Transduction Developmental Biology |
| Zdroj: | Mechanisms of Development. 119(2):177-190 |
| ISSN: | 0925-4773 |
| DOI: | 10.1016/s0925-4773(02)00359-3 |
| Popis: | We have identified a novel Tolloid-like metalloprotease, called Xolloid-related (Xlr), that is expressed during early Xenopus development. Transcripts for xlr are localized to the marginal zone of mid-gastrulae and are most abundant in ventral and lateral sectors. At neurula stages xlr is strongly expressed around the blastopore and in the pharyngeal endoderm, and more weakly expressed throughout the ventral half of the embryo. Transcripts are detected in the nervous system, particularly the hindbrain and spinal cord, and tailbud of tailbud stage embryos, with weaker expression in the anterior nervous system, otic vesicle, heart, and pronephric duct. Transcription of xlr is increased by BMP4 and decreased by Noggin and tBR, indicating that xlr is regulated by BMP signalling. Injection of xlr mRNA inhibits dorsoanterior development and the dorsal axis inducing ability of coinjected chordin, but not noggin or tBR, mRNA. Xlr conditioned media cleaves Chordin in vitro, indicating that this protease may regulate the availability of Chordin in vivo. |
| Databáze: | OpenAIRE |
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