Antigen presentation and tumor cytotoxicity by interferon-gamma-treated microglial cells
| Autor: | Karl Frei, Cornelia Schwerdel, Adriano Fontana, Peter Groscurth, Christine Siepl, Stefan Bodmer |
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| Rok vydání: | 1987 |
| Předmět: |
Cytotoxicity
Immunologic Male Immunology Antigen presentation Antigen-Presenting Cells Macrophage-1 Antigen Complement receptor Receptors Fc Carboxylesterase Interferon-gamma Mice Antigen Colony-Stimulating Factors Immunology and Allergy Macrophage Animals Antigen-presenting cell Interleukin 3 Mice Inbred ICR Phagocytes CD40 biology Tumor Necrosis Factor-alpha Histocompatibility Antigens Class II Cell biology Receptors Complement Endotoxins Mice Inbred C57BL Antigens Surface biology.protein Tumor necrosis factor alpha Female Interleukin-3 Carboxylic Ester Hydrolases Neuroglia Cell Division |
| Zdroj: | European journal of immunology. 17(9) |
| ISSN: | 0014-2980 |
| Popis: | In this study microglial cells isolated from brain cell cultures of newborn mice were characterized and investigated for morphology, their responses to growth factors and their functional properties. The microglial cells were phagocytic, contained nonspecific esterase activity and expressed Fc (IgG1/2b) and type-3 complement receptors. Scanning electron microscopy revealed that in analogy to brain tissue two types of microglial cells are present in the cultures: the ameboid and the ramified type which both display similar appearance by transmission electron microscopy. Interleukin 3 and the granulocyte-macrophage colony-stimulating factor were potent growth factors for the cultured microglial cells. The cells were negative for class II antigens (Ia) of the major histocompatibility antigen complex. However, upon treatment with interferon-gamma (IFN-gamma) microglial cells became Ia+ and functioned as antigen-presenting cells when tested on ovalbumin-specific Ia-restricted helper T cells. Furthermore, microglial cells exposed to IFN-gamma and endotoxin developed tumor cell cytotoxicity and produced tumor necrosis factor alpha. Taken together, microglial cells share the characteristics of cells of the macrophage lineage. |
| Databáze: | OpenAIRE |
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