Clear cell carcinoma of the ovary: characterization of its CD44 isoform repertoire
| Autor: | Lorna Rodriguez-Rodriguez, Darlene G. Gibbon, Clara Mesonero, Jean-Luc Miller Watelet, Inés Sancho-Torres |
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| Rok vydání: | 2000 |
| Předmět: |
Adult
Pathology medicine.medical_specialty Ovary Disease-Free Survival medicine Humans Protein Isoforms Neoplasm Metastasis Aged Ovarian Neoplasms biology business.industry CD44 Obstetrics and Gynecology Middle Aged medicine.disease Primary tumor Immunohistochemistry Alternative Splicing medicine.anatomical_structure Hyaluronan Receptors Oncology Clear cell carcinoma biology.protein Adenocarcinoma Female Neoplasm Recurrence Local Ovarian cancer business Clear cell Adenocarcinoma Clear Cell |
| Zdroj: | Gynecologic oncology. 79(2) |
| ISSN: | 0090-8258 |
| Popis: | Objective. CD44 is a cell surface receptor implicated in tumor metastases. We have previously shown that there is a loss of CD44 splice control in clear cell carcinoma (CCCa) of the ovary. Our aim is to characterize the expression of CD44-3v, -5v, -7v, and -10v in clear cell ovarian tumors and to determine their prognostic value. Methods. Twenty-two cases of ovarian CCCa were studied for CD44-3v, -5v, -7v, and -10v expression by immunocytochemistry. Results. The primary tumors showed expression of CD44-3v, -5v, -7v, and -10v in 44, 55, 61, and 39% of the cases, respectively. We were able to compare the expression of CD44 in the primary tumor and metastatic sites from the same patient in 7 cases (metastatic sites n = 16). We observed decreased immunoreactivity of CD44-3v, -5v, -7v, and -10v in 67, 100, 93, and 92% of the sites, respectively. CD44-3v and -10v expression was absent in 100% of the nonaffected contralateral ovaries while -7v and -10v were expressed in 1/11 (9%) of them. When CD44-10v was not expressed in the primary tumor, only 18% of the women recurred or died of disease; in contrast, of the cases where it was present, 71% of the women recurred or died of disease ( P = 0.049). Conclusions. There is aberrant alternative mRNA splicing in the development of CCCa of the ovary when compared to the contralateral nonaffected ovary. The expression of CD44-10v correlates with survival. Larger series are needed to further understand the role of CD44 isoforms in ovarian cancer metastases. |
| Databáze: | OpenAIRE |
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