Twist1 is an independent prognostic factor of esophageal squamous cell carcinoma and associated with its epithelial-mesenchymal transition
| Autor: | Songying Han, Jeong Hoon Kim, In-Gu Do, Seok-Hyung Kim, Chang Ohk Sung, Sung Hee Um, Young-Hyeh Ko, Keun-Woo Lee |
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| Rok vydání: | 2010 |
| Předmět: |
Oncology
Male medicine.medical_specialty animal structures Epithelial-Mesenchymal Transition Esophageal Neoplasms Immunoenzyme Techniques Twist transcription factor Esophagus Surgical oncology Internal medicine Carcinoma medicine Biomarkers Tumor Tumor Cells Cultured Humans Epithelial–mesenchymal transition RNA Messenger Neoplasm Staging Oligonucleotide Array Sequence Analysis business.industry Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Twist-Related Protein 1 Cancer Nuclear Proteins Esophageal cancer Middle Aged medicine.disease Prognosis Survival Rate medicine.anatomical_structure Tumor progression Case-Control Studies Lymphatic Metastasis Cancer research Carcinoma Squamous Cell Surgery Female business Follow-Up Studies |
| Zdroj: | Annals of surgical oncology. 19(1) |
| ISSN: | 1534-4681 |
| Popis: | Twist1 is known to be involved in epithelial-mesenchymal transition (EMT) and tumor progression. However, its specific role is different depending on the type of cancer. The functional role and clinical significance of Twist1 in esophageal squamous cell carcinoma (ESCC) have been rarely studied.We examined Twist1 expression in a total of 199 samples using immunohistochemistry and real-time reverse-transcription polymerase chain reaction (RT-PCR). We also studied the association of Twist1 with EMT of ESCC using tissue samples and in vitro models. In addition, we examined the changes in the whole-genome expression profiles of two ESCC cell lines after inducing upregulation of Twist1.Twist1 expression was increased in ESCC compared with that of normal esophagus (P 0.01). We found that expression of Twist1 was strongly associated with poor prognosis on both univariate analysis [relative risk (RR) = 3.019, P = 0.000] and multivariate analysis (RR = 3.131, P = 0.000). In addition, we found that Twist1 expression was strongly correlated with EMT in ESCC tissues. Upregulation of Twist1 induced other EMT inducers in all three esophageal cancer cell lines investigated (TE1, TE8, and TE10). Twist1 also enhanced the migratory and invasive abilities of all three cell lines. Finally, microarray analysis of gene expression in the three ESCC cell lines revealed that Twist1 upregulation affected the expression of mainly cytoskeleton and extracellular matrix-related genes, which are directly related to cell morphology and movement.Our study indicates that Twist1 is associated with EMT of esophageal squamous cell carcinoma and may be used as a diagnostic and prognostic biomarker in ESCC. |
| Databáze: | OpenAIRE |
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