Oncogenic Kit signalling on the Golgi is suppressed by blocking secretory trafficking with M-COPA in gastrointestinal stromal tumours
| Autor: | Hiroyasu Esumi, Keita Yonekura, Toshirou Nishida, Keita Horikawa, Tsuyoshi Takahashi, Yasutaka Tasaki, Isamu Shiina, Ryo Abe, Takatsugu Murata, Yuuki Obata, Kyohei Suzuki |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cancer Research Gastrointestinal Stromal Tumors Pyridines Golgi Apparatus Naphthols Protein tyrosine phosphatase Endoplasmic Reticulum 03 medical and health sciences symbols.namesake 0302 clinical medicine Cell Line Tumor medicine Humans Phosphorylation neoplasms Oncogene Proteins Microscopy Confocal Chemistry Autophosphorylation Imatinib Golgi apparatus digestive system diseases Protein Transport Proto-Oncogene Proteins c-kit 030104 developmental biology Oncology Protein kinase domain 030220 oncology & carcinogenesis Mutation Cancer research symbols Tyrosine Signal transduction Tyrosine kinase Signal Transduction medicine.drug |
| Zdroj: | Cancer Letters. 415:1-10 |
| ISSN: | 0304-3835 |
| DOI: | 10.1016/j.canlet.2017.11.032 |
| Popis: | Most gastrointestinal stromal tumours (GISTs) are caused by constitutively active mutations in Kit tyrosine kinase. The drug imatinib, a specific Kit inhibitor, improves the prognosis of metastatic GIST patients, but these patients become resistant to the drug by acquiring secondary mutations in the Kit kinase domain. We recently reported that a Kit mutant causes oncogenic signals only on the Golgi apparatus in GISTs. In this study, we show that in GIST, 2-methylcoprophilinamide (M-COPA, also known as "AMF-26"), an inhibitor of biosynthetic protein trafficking from the endoplasmic reticulum (ER) to the Golgi, suppresses Kit autophosphorylation at Y703/Y721/Y730/Y936, resulting in blockade of oncogenic signalling. Results of our M-COPA treatment assay show that Kit Y703/Y730/Y936 in the ER are dephosphorylated by protein tyrosine phosphatases (PTPs), thus the ER-retained Kit is unable to activate downstream molecules. ER-localized Kit Y721 is not phosphorylated, but not due to PTPs. Importantly, M-COPA can inhibit the activation of the Kit kinase domain mutant, resulting in suppression of imatinib-resistant GIST proliferation. Our study demonstrates that Kit autophosphorylation is spatio-temporally regulated and may offer a new strategy for treating imatinib-resistant GISTs. |
| Databáze: | OpenAIRE |
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