New Selective Media for the Isolation ofStreptococcus hemolyticus
| Autor: | Yasuzo Nakamizo, Maroto Sato |
|---|---|
| Rok vydání: | 1972 |
| Předmět: |
Adult
Male food.ingredient Adolescent Scarlet Fever Biology medicine.disease_cause Microbiology Agar plate chemistry.chemical_compound food medicine Humans Agar Child Streptococcus General Medicine Saponins Haemolysis medicine.disease Urease Camphor Culture Media stomatognathic diseases chemistry Charcoal Child Preschool Streptococcus pyogenes Pharynx Scarlet fever Sodium azide Female Staphylococcus |
| Zdroj: | American Journal of Clinical Pathology. 57:228-235 |
| ISSN: | 1943-7722 0002-9173 |
| Popis: | A new enrichment broth (E.U.B.) and a modified agar medium (M.H.A) were devised for the purpose of obtaining β -streptococci selectively in increasing numbers from throat swabs. The new broth replaces animal blood with activated charcoal, uses a high concentration of NaCl (3%), saponin, DL-camphor, and sodium azide as inhibitory agents against contaminants, and includes urease as a stimulating agent for β -streptococci. Urea is added to the modified agar medium to promote the growth of β -streptococci, and crystal violet assists by inhibiting the proliferation of staphylococci. The incidences of positive cultures of β -streptococci from 1,225 throat swabs from 112 patients with scarlet fever were 47.2% (579) with the new media and 23.6% (290 cultures) by the routine anaerobic blood agar method. Furthermore, β -streptococci from 11 throat swabs collected from patients after antibiotic therapy had been instituted could be isolated only by the new culture method. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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