| Popis: |
We report the nucleotide and deduced amino acid sequence for electric organ antigens (3), including a frog postsynaptic antigen an intermediate filament (IF) gene from the elasmobranch that is recognized by the IF monoclonal antibody (4), has not Torpedo californica. The clone was isolated from a Xgtl 1 cDNA been determined. expression library encoding transcripts from the electric organ and the sequence was obtained from both DNA strands. The deduced protein sequence is highly homologous to other IF REFERENCES proteins in the rod and coil demains, especially desmin and vimentin (1). Western blotting showed that a mon6clonal antibody 1. Weber2K. and Geisler,N. (1985) Annals New YorkAcademyofSciences 455, that recognizes all classes of IFs (2) recognizes a ,B-galactosidlase 2. Pruss et al. (1981) Cell 27, 419-428. fusion protein that encodes the sequences of the full length clone 3. Froehner,S. (1984) J. Cell Biol. 99, 88-96. (data not shown). The relationship between our clone and other 4. Burden,S. (1982) J. Cell Biol. 94, 521-530. |
