Effect of self-assembling peptide, chondrogenic factors, and bone marrow-derived stromal cells on osteochondral repair
| Autor: | John D. Kisiday, Alan J. Grodzinsky, Eric J. Vanderploeg, Rachel E. Miller, Dora J. Ferris, Myra F. Barrett, Christina M. Lee, David D. Frisbie |
|---|---|
| Přispěvatelé: | Massachusetts Institute of Technology. Center for Biomedical Engineering, Massachusetts Institute of Technology. Department of Biological Engineering, Miller, Rachel Elizabeth, Grodzinsky, Alan J., Vanderploeg, Eric J. |
| Rok vydání: | 2010 |
| Předmět: |
Cartilage
Articular Stromal cell Biomedical Engineering Bone Marrow Cells Mesenchymal Stem Cell Transplantation Bone marrow-derived mesenchymal stem cells Dexamethasone Article Transforming Growth Factor beta1 Andrology Self-assembling peptide scaffold Rheumatology Tissue engineering In vivo medicine Animals Regeneration Orthopedics and Sports Medicine Insulin-Like Growth Factor I Aggrecan Rabbit model Tissue Engineering Tissue Scaffolds Chemistry Cartilage Synovial Membrane Hydrogels Chondrogenesis Radiography medicine.anatomical_structure Immunology Female Rabbits Bone marrow Immunostaining |
| Zdroj: | PMC |
| ISSN: | 1063-4584 |
| DOI: | 10.1016/j.joca.2010.09.004 |
| Popis: | Objective The goal of this study was to test the ability of an injectable self-assembling peptide (KLD) hydrogel with or without chondrogenic factors (CF) and allogeneic bone marrow stromal cells (BMSCs) to stimulate cartilage regeneration in a full-thickness, critically-sized, rabbit cartilage defect model in vivo. We used CF treatments to test the hypotheses that CF would stimulate chondrogenesis and matrix production by cells migrating into acellular KLD (KLD + CF) or by BMSCs delivered in KLD (KLD + CF + BMSCs). Design Three groups were tested against contralateral untreated controls: KLD, KLD + CF, and KLD + CF +BMSCs, n = 6–7. Transforming growth factor-β1 (TGF-β1), dexamethasone, and insulin-like growth factor-1 (IGF-1) were used as CF pre-mixed with KLD and BMSCs before injection. Evaluations included gross, histological, immunohistochemical and radiographic analyses. Results KLD without CF or BMSCs showed the greatest repair after 12 weeks with significantly higher Safranin-O, collagen II immunostaining, and cumulative histology scores than untreated contralateral controls. KLD + CF resulted in significantly higher aggrecan immunostaining than untreated contralateral controls. Including allogeneic BMSCs + CF markedly reduced the quality of repair and increased osteophyte formation compared to KLD-alone. Conclusions These data show that KLD can fill full-thickness osteochondral defects in situ and improve cartilage repair as shown by Safranin-O, collagen II immunostaining, and cumulative histology. In this small animal model, the full-thickness critically-sized defect provided access to the marrow, similar in concept to abrasion arthroplasty or spongialization in large animal models, and suggests that combining KLD with these techniques may improve current practice. National Institutes of Health (U.S.) (National Institute for Biomedical Imaging and Bioengineering (U.S.) Grant EB003805) American Society for Engineering Education. National Defense Science and Engineering Graduate Fellowship National Science Foundation (U.S.). Graduate Research Fellowship National Institutes of Health (U.S.) (Grant EB003805) National Institutes of Health (U.S.) (Grant AR33236) Arthritis Foundation (Postdoctoral Fellowship) |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect