片側末梢投与されたA型ボツリヌス毒素は動物モデルにおいて両側三叉神経節に局在する
| Autor: | Akihito Yamamoto, Yumiko Yamamoto, Daisuke Ikutame, Masamitsu Oshima, Kazuo Okura, Shaista Afroz, Yoshizo Matsuka, Huijiao Yan, Resmi Raju, Otto Baba, Arief Waskitho, Tsuyoshi Morita, Fumiya Kano, Swarnalakshmi Raman |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
trigeminal ganglion Health Toxicology and Mutagenesis Stimulation Pharmacology Toxicology Article Rats Sprague-Dawley Trigeminal ganglion Infraorbital nerve Mice medicine Animals botulinum toxin Botulinum Toxins Type A Trigeminal nerve neuropathic pain Mice Inbred ICR business.industry Botulinum toxin Rats Disease Models Animal Sensory Ganglion Peripheral nerve injury Neuropathic pain Neuralgia Medicine Female business medicine.drug |
| Zdroj: | Toxins, Vol 13, Iss 704, p 704 (2021) Toxins Volume 13 Issue 10 |
| ISSN: | 2072-6651 |
| Popis: | Peripheral nerve injury leads to sensory ganglion hyperexcitation, which increases neurotransmitter release and neuropathic pain. Botulinum toxin type A (BoNT/A) regulates pain transmission by reducing neurotransmitter release, thereby attenuating neuropathic pain. Despite multiple studies on the use of BoNT/A for managing neuropathic pain in the orofacial region, its exact mechanism of transport remains unclear. In this study, we investigated the effects of BoNT/A in managing neuropathic pain in two different animal models and its transport mechanism in the trigeminal nerve. Intraperitoneal administration of cisplatin induced bilateral neuropathic pain in the orofacial region, reducing the head withdrawal threshold to mechanical stimulation. Unilateral infraorbital nerve constriction (IONC) also reduced the ipsilateral head withdrawal threshold to mechanical stimulation. Unilateral peripheral administration of BoNT/A to the rat whisker pad attenuated cisplatin-induced pain behavior bilaterally. Furthermore, contralateral peripheral administration of BoNT/A attenuated neuropathy-induced behavior caused by IONC. We also noted the presence of BoNT/A in the blood using the mouse bioassay. In addition, the Alexa Fluor-488-labeled C-terminal half of the heavy chain of BoNT/A (BoNT/A-Hc) was localized in the neurons of the bilateral trigeminal ganglia following its unilateral administration. These findings suggest that axonal and hematogenous transport are involved in the therapeutic effects of peripherally administered BoNT/A in the orofacial region. |
| Databáze: | OpenAIRE |
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