High-Dose Intravenous Immunoglobulin Treatment Activates Complement In Vivo
Autor: | T E, Mollnes, K, Høgåsen, C, De Carolis, E, Vaquero, E W, Nielsen, L, Fontana, R, Perricone |
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Rok vydání: | 1998 |
Předmět: |
Immunoglobulin A
Abortion Habitual Immunology Dose-Response Relationship Immunologic Immunoglobulins Complement C5a Complement Membrane Attack Complex Complement C1 Inactivator Proteins Immunoglobulin G Classical complement pathway Pregnancy Recurrence In vivo Humans Medicine Complement Activation Serum Albumin Glycoproteins Autoimmune disease Complement C3 Convertase Alternative Pathway Complement Inactivator Proteins biology business.industry Complement C1q Pregnancy Outcome Immunoglobulins Intravenous Complement C4 Blood Proteins Complement C3 General Medicine medicine.disease Peptide Fragments In vitro Receptors Complement Complement system Immunoglobulin M Complement C3c Complement C3b biology.protein Female business |
Zdroj: | Scandinavian Journal of Immunology. 48:312-317 |
ISSN: | 1365-3083 0300-9475 |
Popis: | Several complement modulating effects of high-dose intravenous immunoglobulins (IVIG) have been proposed from in vitro studies and experimental animal models. However, the in vivo effects of IVIG on plasma complement in humans are yet not known. We have investigated the in vivo effects of IVIG on complement in seven women with unexplained recurrent spontaneous abortion who were without evidence of autoimmune disease. Samples were obtained before and after the very first infusion of IVIG. There was a marked increase in immunoglobulin G (IgG) from (median and range) 12.4 (9.4-15.9) to 26.8 (22.4-30.0) g/l but no change in immunoglobulin A (IgA) or immunoglobulin M (IgM). A significantly increased complement activation was demonstrated using neoepitope-specific enzyme immunoassays to the activation products C3bc (median increased from 9.8 to 31.2 AU/ml), Bb (0.66-1.66 g/ml), C5a (10.5-12.7 ng/ml), and TCC (0.81-2.19 AU/ml) (P = 0.015 for all). There were no changes in antigenic concentrations of individual complement components or regulators (C1q, C4, C3, C1-inhibitor, C4b-binding protein) and no decrease in complement haemolytic activity (classical and alternative CH50), which were all within the normal range. The classical pathway activation products C1rs/C1-inhibitor complexes, C4bc and C4d were elevated in all patients before IVIG treatment and did not change significantly during treatment. In conclusion, IVIG induced a significant activation of complement in vivo. |
Databáze: | OpenAIRE |
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