HIGH MOLECULAR WEIGHT VASOPRESSIN: DETECTION OF A LARGE AMOUNT IN THE PLASMA OF A PATIENT
| Autor: | Paul Franchimont, Jean-Jacques Legros, Simon Smitz, M. Le Maire |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
medicine.medical_specialty
Vasopressin Lung Neoplasms Endocrinology Diabetes and Metabolism Radioimmunoassay Peptide hormone Inappropriate ADH Syndrome chemistry.chemical_compound Endocrinology Internal medicine Mole medicine Humans Carcinoma Small Cell Aged medicine.disease Trypsin Arginine Vasopressin Molecular Weight chemistry Sephadex Syndrome of inappropriate antidiuretic hormone secretion Urea medicine.drug |
| Zdroj: | Clinical Endocrinology. 23:379-384 |
| ISSN: | 1365-2265 0300-0664 |
| Popis: | Using a sensitive and specific radioimmunoassay for arginine-vasopressin, we have searched for the presence of high molecular weight (HMW) vasopressin in the plasma of a patient with the syndrome of inappropriate antidiuretic hormone secretion (SIADH) and an oat cell carcinoma of the lung. After incubation in 8 mol/l urea, one millilitre of the plasma from this patient was fractionated on a Sephadex G-50 column and the immunoreactive vasopressin content was evaluated before and after trypsin treatment of the eluted fractions. A wide peak of apparent mol. wt. 2500-6000 daltons was revealed only after tryptic digestion of each fraction. This peak contained the equivalent of 1900 pg of vasopressin. A tryptic digest of this peak, rechromatographed on Sephadex G-25, gave two small peptides the major one eluting at a position identical to vasopressin. These results demonstrate the presence of a large amount of HMW vasopressin in the plasma of a patient with SIADH and oat cell carcinoma of the lung. |
| Databáze: | OpenAIRE |
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