Reactive aldehyde-scavenging enzyme activities in atherosclerotic plaques of cigarette smokers and nonsmokers
| Autor: | Sante Ucchino, Franco Cuccurullo, Carmine Di Ilio, Maria Adele Giamberardino, Giuliano Ciofani, Domenico Lapenna |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Carotid Artery Diseases
Male medicine.medical_specialty Down-Regulation Aldehyde dehydrogenase medicine.disease_cause Severity of Illness Index Isozyme Aldehyde Dehydrogenase 1 Family Lipid peroxidation chemistry.chemical_compound Aldehyde Reductase Internal medicine Humans Medicine Aged Glutathione Transferase ALDH2 Aldose reductase biology business.industry Aldehyde Dehydrogenase Mitochondrial Smoking Retinal Dehydrogenase Glutathione Aldehyde Dehydrogenase Middle Aged Prognosis Plaque Atherosclerotic Isoenzymes Oxidative Stress Carotid Arteries Endocrinology Glutathione S-transferase chemistry Biochemistry biology.protein Female Lipid Peroxidation Cardiology and Cardiovascular Medicine business Biomarkers Oxidative stress |
| Zdroj: | Atherosclerosis. 238:190-194 |
| ISSN: | 0021-9150 |
| DOI: | 10.1016/j.atherosclerosis.2014.11.028 |
| Popis: | Objective : To investigate enzymatic reactive aldehyde-scavenging enzyme capacity together with lipid peroxidation as expression of oxidative stress in atherosclerotic plaques of cigarette smokers and nonsmokers. Methods : We have assessed specific enzymatic activities of class 1, 2, and 3 aldehyde dehydrogenase (ALDH1, ALDH2, and ALDH3, respectively), glutathione S -transferase (isozyme A4-4, GSTA4-4), and aldose reductase (AR), namely the major reactive aldehyde-scavenging enzymes, together with lipid peroxidation, i.e., fluorescent damage products of lipid peroxidation (FDPL), in carotid atherosclerotic plaques surgically removed from 17 cigarette smokers and 17 nonsmokers. Results : The enzymatic activities of ALDH1 plus ALDH2, ALDH3, GSTA4-4, and AR were significantly lower in the atherosclerotic plaques of smokers than in those of nonsmokers, while plaque FDPL levels were significantly higher in the smokers than in the nonsmokers. The amount of cigarette smoking was correlated inversely with the aforementioned plaque enzymatic activities and directly with plaque FDPL content. Plaque FDPL levels were inversely correlated with plaque enzymatic activities in smokers and nonsmokers. The degree of carotid atherosclerotic stenosis, as expression of atherosclerosis severity, was correlated inversely with plaque enzymatic activities and directly with plaque FDPL levels in smokers and nonsmokers; moreover, the degree of carotid stenosis was directly correlated with the amount of cigarette smoking. Conclusion : atherosclerotic lesions of cigarette smokers are endowed with a depressed enzymatic reactive aldehyde-scavenging capacity eventually favoring oxidative stress and the severity of atherosclerosis. |
| Databáze: | OpenAIRE |
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