Adenovirus-mediated angiopoietin-1 gene therapy enhances skin flap survival
| Autor: | Maria Siemionow, Joe Scharpf, Howard Jung, Raffi Gurunluoglu |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Male
medicine.medical_specialty medicine.medical_treatment Genetic enhancement Dermatologic Surgical Procedures Green Fluorescent Proteins Skin flap Surgical Flaps Adenoviridae Rats Sprague-Dawley Angiopoietin 1 Gene Angiopoietin-1 medicine Animals In patient Therapeutic angiogenesis Control treatment integumentary system Abdominal skin business.industry Graft Survival Genetic Therapy Microsurgery Rats Surgery Luminescent Proteins Indicators and Reagents business |
| Zdroj: | Microsurgery. 23:374-380 |
| ISSN: | 1098-2752 0738-1085 |
| Popis: | This experimental study investigates the feasibility of pretreating the abdominal skin of a rat with subdermal injections of adenovirus encoding angiopoietin-1 in order to improve postoperative survival of the skin flap. An epigastric skin flap was used as the model in this study. Rats received subdermal injections of adenovirus encoding either angiopoietin-1 (treatment group) or green fluorescent protein (treatment control), or they received no treatment (control group). Subdermal injections were made 2 days prior to surgery, and skin flap survival was assessed 7 days afterwards as a percentage of necrotic area over total skin flap area. The treatment group which received adenovirus-mediated angiopoietin-1 had a median percent necrotic area of 11.01%, a significant decrease from the control group, which had a median percent necrotic area of 32.24% (P < 0.001). The results of this study suggest the possibility of using adenovirus-mediated angiopoietin-1 gene therapy to promote therapeutic angiogenesis in patients who undergo reconstructive procedures. © 2003 Wiley-Liss, Inc. MICROSURGERY 23:374–380 2003 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text