Skeletal muscle membrane lipid composition is related to adiposity and insulin action
| Autor: | Michael R Milner, Louise A. Baur, Clifton Bogardus, David A. Pan, Stephen Lillioja, Adamandia D. Kriketos, Leonard H Storlien |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Blood Glucose Fatty Acid Desaturases Male medicine.medical_specialty Biopsy medicine.medical_treatment Phospholipid Adipose tissue Calorimetry Biology Body Mass Index Membrane Lipids chemistry.chemical_compound Delta-5 Fatty Acid Desaturase Insulin resistance Internal medicine medicine Humans Insulin Obesity Muscle Skeletal Phospholipids chemistry.chemical_classification Fatty Acids Arizona Fatty acid Skeletal muscle General Medicine Glucose clamp technique medicine.disease Endocrinology medicine.anatomical_structure Adipose Tissue chemistry Glucose Clamp Technique Indians North American Regression Analysis Research Article Polyunsaturated fatty acid |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0021-9738 |
| DOI: | 10.1172/jci118350 |
| Popis: | The cellular basis of insulin resistance is still unknown; however, relationships have been demonstrated between insulin action in muscle and the fatty acid profile of the major membrane structural lipid (phospholipid). The present study aimed to further investigate the hypothesis that insulin action and adiposity are associated with changes in the structural lipid composition of the cell. In 52 adult male Pima Indians, insulin action (euglycemic clamp), percentage body fat (pFAT; underwater weighing), and muscle phospholipid fatty acid composition (percutaneous biopsy of vastus lateralis) were determined. Insulin action (high-dose clamp; MZ) correlated with composite measures of membrane unsaturation (% C20-22 polyunsaturated fatty acids [r= 0.463, P < 0.001], unsaturation index [r= -0.369, P < 0.01]), a number of individual fatty acids and with delta5 desaturase activity (r= 0.451, P < 0.001). pFAT (range 14-53%) correlated with a number of individual fatty acids and delta5 desaturase activity (r= -0.610, P < 0.0001). Indices of elongase activity (r= -0.467, P < 0.001), and delta9 desaturase activity (r= 0.332, P < 0.05) were also related to pFAT but not insulin action. The results demonstrate that delta5 desaturase activity is independently related to both insulin resistance and obesity. While determining the mechanisms underlying this relationship is important for future investigations, strategies aimed at restoring "normal" enzyme activities, and membrane unsaturation, may have therapeutic importance in the "syndromes of insulin resistance." |
| Databáze: | OpenAIRE |
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