Rotavirus G2P[4] Detection in Fresh Vegetables and Oysters in Mexico City
| Autor: | Elsa Irma Quiñones-Ramírez, Carlos Vázquez-Salinas, Iván Natividad-Bonifacio, Carolina Quiroz-Santiago, Blanca Lilia Barrón-Romero |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Rotavirus
Oyster Genotype viruses Food Contamination medicine.disease_cause Microbiology Rotavirus Infections Virus biology.animal Vegetables medicine Animals Ingestion Mexico Genotyping Shellfish biology Ostreidae Virology Reverse transcription polymerase chain reaction Diarrhea medicine.symptom Food Science |
| Zdroj: | Journal of Food Protection. 77:1953-1959 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x.jfp-13-426 |
| Popis: | Rotaviruses are the principal cause of dehydration caused by diarrhea in children younger than 2 years of age. Although these viral infections have mainly been associated with ingestion of fecally contaminated food and water, few studies have addressed the presence of the virus in food that is consumed raw or slightly cooked. In this work, 30 oyster samples and 33 vegetable samples were examined for the presence of rotavirus genotypes to evaluate their potential to produce gastrointestinal infections. The rotaviruses were identified by reverse transcriptase PCR amplification of the VP7 gene. G and P genotyping was also performed by reverse transcriptase PCR, with a detection sensitivity of up to 15 PFU/ml. Rotaviruses were found in 17 (26.9%) of 63 samples (10 oysters and 7 vegetables). The G2 genotype was found in 11 (64.7%) of 17 of the rotavirus strains, and 16 (94.1%) of 17 had the P[4] genotype. The combined genotypes found most frequently were G2P[4] (10 [58.82%] of 17), GNTP[4] (6 [35.29%] of 17), and G2P[NT] (1 [5.8%] of 17). |
| Databáze: | OpenAIRE |
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