The role of EMMPRIN expression in ovarian epithelial carcinomas
| Autor: | Shuang Zhao, Li-jun Xiao, Yasuo Takano, Yang Zhao, Shuo Chen, Wen-Feng Gou, Zhe-Feng Niu, Hua-chuan Zheng |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Stromal cell
Carcinogenesis EMMPRIN Biology Carcinoma Ovarian Epithelial Metastasis chemistry.chemical_compound Ovarian carcinoma Cell Line Tumor Report Survivin medicine Humans Neoplasms Glandular and Epithelial RNA Messenger Molecular Biology Aged Ovarian Neoplasms Cell Biology Middle Aged medicine.disease Prognosis Molecular biology Immunohistochemistry Vascular endothelial growth factor Gene Expression Regulation Neoplastic Phenotype ovarian cancer chemistry Basigin Gene Knockdown Techniques cellular phenotypes Cancer cell Multivariate Analysis Cancer research Female progression Ovarian cancer Developmental Biology |
| Zdroj: | Cell Cycle |
| ISSN: | 1551-4005 |
| Popis: | Purpose: Extracellular matrix metalloproteinase inducer (EMMPRIN) was reported to involve in the invasion and metastasis of malignancies by regulating the expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs) in stromal and cancer cells. The study aimed to clarify the role of EMMPRIN expression in tumorigenesis and progression of ovarian epithelial carcinomas. Methods: EMMPRIN siRNA were transfected into ovarian carcinoma cells with the phenotypes and their related molecules examined. EMMPRIN expression was determined in ovarian normal tissue, benign and borderline tumors, and epithelial carcinomas by real-time PCR, western blot, and immunohistochemisty. Results: EMMPRIN siRNA treatment resulted in a lower growth, G1 arrest, apoptotic induction, decreased migration, and invasion. The transfectants showed reduced expression of Wnt5a, Akt, p70s6k, Bcl-xL, survivin, VEGF, and MMP-9 than mock and control cells at both mRNA and protein levels. According to real-time PCR and western blot, EMMPRIN mRNA or protein level was higher in ovarian borderline tumor and carcinoma than normal ovary and benign tumors (P < 0.05), and positively correlated with dedifferentiation and FIGO staging (P < 0.05). Immuhistochemically, EMMPRIN expression was positively correlated with FIGO staging, dedifferentiation, Ki-67 expression, the lower cumulative and relapse-free survival rate (P < 0.05). Conclusions: Upregulated expression of EMMPRIN protein and mRNA might be involved in the pathogenesis, differentiation, and progression of ovarian carcinomas, possibly by modulating cellular events, such as proliferation, cell cycle, apoptosis, migration, and invasion. |
| Databáze: | OpenAIRE |
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