Characterization of the endocannabinoid system in subcutaneous adipose tissue in periparturient dairy cows and its association to metabolic profiles

Autor: L Livshitz, Shiran Udi, Yishai Levin, Joseph Tam, Alina Nemirovski, M. Zachut, Uzi Moallem, G. Kra
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
Proteomics
Blood Glucose
Physiology
medicine.medical_treatment
Adipose tissue
Gene Expression
lcsh:Medicine
Pathology and Laboratory Medicine
Biochemistry
Oleoylethanolamide
chemistry.chemical_compound
Database and Informatics Methods
0302 clinical medicine
Endocrinology
Weight loss
Reproductive Physiology
Pregnancy
Lactation
Medicine and Health Sciences
Insulin
lcsh:Science
Immune Response
Multidisciplinary
Proteomic Databases
Hydrolysis
Fatty Acids
Postpartum Period
Chemical Reactions
Eukaryota
Plants
Legumes
Lipids
Chemistry
Dairying
medicine.anatomical_structure
Physical Sciences
Metabolome
Female
medicine.symptom
Research Article
medicine.medical_specialty
Lipolysis
Immunology
Subcutaneous Fat
030209 endocrinology & metabolism
Biology
Research and Analysis Methods
Glucagon
03 medical and health sciences
Signs and Symptoms
Diagnostic Medicine
Internal medicine
Weight Loss
medicine
Genetics
Animals
RNA
Messenger
Inflammation
Endocrine Physiology
lcsh:R
Organisms
Peas
Biology and Life Sciences
Diet
Oxidative Stress
030104 developmental biology
Biological Databases
chemistry
Cattle
lcsh:Q
Postpartum period
Endocannabinoids
Zdroj: PLoS ONE, Vol 13, Iss 11, p e0205996 (2018)
PLoS ONE
ISSN: 1932-6203
Popis: Adipose tissue (AT) plays a major role in metabolic adaptations in postpartum (PP) dairy cows. The endocannabinoid (eCB) system is a key regulator of metabolism and energy homeostasis; however, information about this system in ruminants is scarce. Therefore, this work aimed to assess the eCB system in subcutaneous AT, and to determine its relation to the metabolic profile in peripartum cows. Biopsies of AT were performed at 14 d prepartum, and 4 and 30 d PP from 18 multiparous peripartum cows. Cows were categorized retrospectively according to those with high body weight (BW) loss (HWL, 8.5 ± 1.7% BW loss) or low body weight loss (LWL, 2.9 ± 2.5% BW loss) during the first month PP. The HWL had higher plasma non-esterified fatty acids and a lower insulin/glucagon ratio PP than did LWL. Two-fold elevated AT levels of the main eCBs, N-arachidonoylethanolamine (AEA) and 2-arachidonoylglycerol (2-AG), were found 4 d PP compared with prepartum in HWL, but not in LWL cows. AT levels of the eCB-like molecules oleoylethanolamide, palmitoylethanolamide, and of arachidonic acid were elevated PP compared with prepartum in all cows. The abundance of monoglyceride lipase (MGLL), the 2-AG degrading enzyme, was lower in HWL vs. LWL AT PP. The relative gene expression of the cannabinoid receptors CNR1 and CNR2 in AT tended to be higher in HWL vs. LWL PP. Proteomic analysis of AT showed an enrichment of the inflammatory pathways' acute phase signaling and complement system in HWL vs. LWL cows PP. In summary, eCB levels in AT were elevated at the onset of lactation as part of the metabolic adaptations in PP dairy cows. Furthermore, activating the eCB system in AT is most likely associated with a metabolic response of greater BW loss, lipolysis, and AT inflammation in PP dairy cows.
Databáze: OpenAIRE
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