Chemical Composition and Antimicrobial Effect of Western Ethiopian Propolis

Autor: Tariku Neme Afata, Aman Dekebo
Rok vydání: 2023
Předmět:
Zdroj: Chemistry & Biodiversity. 20
ISSN: 1612-1880
1612-1872
Popis: Propolis or bee glue is commonly named as a natural resinous mixture produced by honeybees (Apis mellifera) from substances collected from parts of plants, buds, and exudate. The result of the ethyl acetate - methanol (3:2) fraction yielded a total of two compounds namely betulinic acid and β-amyrin were isolated from Bodji Dirmaji and Fincha'a district propolis, respectively. The crude ethanolic extract was portioned with the different solvent systems by increasing the polarities in the following order of n-hexane, ethyl acetate, and methanol. Column chromatographic method on normal silica gel was used to isolate the compounds. The structures of the compounds were characterized using 1D NMR techniques. This finding indicates that the ethanolic extract propolis of western Ethiopia may provide a potential source of natural bioactive compounds like triterpenoids that can be relevant in biological and pharmacological applications.Propolis or bee glue is commonly named as a natural resinous mixture produced by honeybees (Apis mellifera) from substances collected from parts of plants, buds, and exudate. The result of the ethyl acetate - methanol (3:2) volume by volume fraction yielded a total of two compounds namely betulinic acid and β-amyrin isolated from Bodji Dirmaji and Fincha'a district propolis, respectively. The crude ethanolic extract was portioned with the different solvent systems by increasing the polarities in the following order of n-hexane, ethyl acetate, and methanol. Column chromatographic method on normal silica gel was used to isolate the compounds. The structures of the compounds were characterized using 1D NMR techniques. The study revealed that western Ethiopian propolis was rich in saponins, tannins, flavonoids, steroids, triterpenes, and glycosides. The antibacterial activity for the isolated compound (betulinic acid) showed the highest inhibition for S.aureus (11.2 ± 1.6), E. coli (17.7 ± 1.1), and A. niger (12.6 ± 1.2) mm.
Databáze: OpenAIRE
Externí odkaz: