Enhancement of 5-iododeoxyuridine-induced endogenous C-type virus activation by polycyclic hydrocarbons: apparent lack of parallelism between enhancement and carcinogenicity
| Autor: | H. Yoshikura, O. Perin-Roussel, Pierre Jacquignon, R. Latarjet, F. Zajdela, F. Périn |
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| Rok vydání: | 1977 |
| Předmět: |
Cancer Research
9 10-Dimethyl-1 2-benzanthracene Drug Evaluation Preclinical Hamster Endogeny Biology In Vitro Techniques Virus Replication Mixed Function Oxygenases chemistry.chemical_compound Cytopathogenic Effect Viral In vivo Idoxuridine Polycyclic Compounds Benzopyrenes Carcinogen Cells Cultured Flavonoids Dose-Response Relationship Drug Virus Activation Drug Synergism Metabolism Kinetics Retroviridae Oncology Biochemistry chemistry Methylcholanthrene Carcinogens Polycyclic Hydrocarbons NADP |
| Zdroj: | Journal of the National Cancer Institute. 58(4) |
| ISSN: | 0027-8874 |
| Popis: | When mouse MLg cells were treated with 3-methylcholanthrene or 7,12-dimethylbenz[alpha]anthracene in the presence of microsomal enzymes and NADPH after 5-iododeoxyuridine (IUDR) treatment, the induction rate of the endogenous C-type virus was increased fivefold to sixfold in comparison with the culture treated with IUDR only. In this reaction, both the microsomal enzymes and NADPH were indispensable. 7,8-Benzoflavone, an inhibitor of the metabolism of hydrocarbons in hamster embryo cultures, inhibited the reaction. For detecting the enhancing activity, the concentration of IUDR for the pretreatment, the concentration of the test products, and the duration of the treatment with the products were important factors. In screening 30 polycyclic hydrocarbons, we were unable to detect a correlation between the in vivo carcinogenicity in the skin and the enhancing activity in the conditions tested. |
| Databáze: | OpenAIRE |
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