HTLV-2 in Central Africa: HTLV-2 subtype B strains similar to those found in Amerindian tribes are endemic in Bakola Pygmies from south Cameroon but not in surrounding Bantus and Baka Pygmies

Autor: Luis Quintana-Murci, Philippe V. Afonso, Sara Calattini, Philippe Mauclère, Antoine Gessain, Laurent Meertens, Renaud Mahieux, Claudia Filippone, Edouard Betsem, Alain Froment, Monique van Beveren, Sabine Plancoulaine
Přispěvatelé: Epidémiologie et Physiopathologie des Virus Oncogènes, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique ( CNRS ), Centre Pasteur du Cameroun, Centre Pasteur du Cameroun-Réseau International des Instituts Pasteur ( RIIP ), Génétique Humaine des Maladies Infectieuses ( Inserm U980 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Faculté de Médecine et des Sciences Biomédicales, Université de Yaoundé I [Yaoundé], Département Sociétés et Santé ( DSS ), Génétique Evolutive Humaine - Human Evolutionary Genetics, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Réseau International des Instituts Pasteur (RIIP), Génétique Humaine des Maladies Infectieuses (Inserm U980), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Yaoundé I, Eco-Anthropologie et Ethnobiologie (EAE), Muséum national d'Histoire naturelle (MNHN)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), BMC, Ed.
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Zdroj: Europe PubMed Central
Retrovirology, Vol 8, Iss Suppl 1, p A82 (2011)
Retrovirology
Retrovirology, BioMed Central, 2011, 8 (Suppl 1), pp.A82
15th International Conference on Human Retroviruses: HTLV and Related Viruses
15th International Conference on Human Retroviruses: HTLV and Related Viruses, Jun 2011, Leuven and Gembloux, Belgium. pp.A82, ⟨10.1186/1742-4690-8-S1-A82⟩
ISSN: 1742-4690
DOI: 10.1186/1742-4690-8-S1-A82⟩
Popis: International audience; Background:Presence and origin of endemic foci of HTLV-2 infection in Africa remain a matter of debate.Material and methods:To better appreciate the epidemiological and molecular determinants of HTLV-2 infection in Central Africa, we performed a survey in 3903 inhabitants of a South Cameroon forest area, including 1051 Bakola Pygmies, 815 Baka Pygmies and 2037 Bantus living in their neighboring. HTLV-1 and HTLV-2 infection was determined by both specific serological (IFA and WB) and molecular (different generic and specific PCR) methods.Results:HTLV-1/2 prevalence was of 3% (117/3903) with 90 HTLV-1 (2.3%) and 27 HTLV-2 (0.7%). Surprisingly, HTLV-2 infection was restricted to Bakola Pygmies (27/1051 2.5%) with no HTLV-2 infection in any of the 2852 Baka or Bantus individuals. In Bakola Pygmies, HTLV-2 seroprevalence increased with age, reaching 6.5% in the elder persons. Ongoing intrafamilial HTLV-2 transmission was evidenced. Lymphoid T cell lines (CD8+ or CD4+, CD25 +) producing HTLV-2 antigens, were established from PBMCs cultures of HTLV-2 infected individuals. Sequences of a 672 nucleotide LTR fragment, obtained from 7 HTLV-2 samples, showed a very high degree of homologies among samples (< 1% nucleotide divergence) but also surprisingly with Amerindian HTLV-2 B strains. Complete sequence (8954 bp) of one isolate confirmed a typical HTLV-2 B strain.Conclusion:This study demonstrates clearly a HTLV-2 endemic population, with ongoing transmission, in Central Africa. Furthermore, it gives insights into several central questions regarding the origin and evolution rate of HTLV-2 and the migrations of infected populations.
Databáze: OpenAIRE
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