Antiviral Drug Ivermectin at Nanomolar Concentrations Inhibits Glycine-Induced Chloride Current in Rat Hippocampal Neurons
| Autor: | Elena I. Solntseva, R. V. Kondratenko, Julia V. Bukanova, Vladimir G. Skrebitsky |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques hippocampus Glycine Action Potentials Pharmacology Hippocampal formation patch clamp Antiviral Agents Chloride General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Receptors Glycine 0302 clinical medicine Desensitization (telecommunications) Chloride Channels Virology medicine Animals Patch clamp Rats Wistar Glycine receptor Cells Cultured Ivermectin Dose-Response Relationship Drug urogenital system Chemistry Pyramidal Cells General Medicine Antiparasitic agent Rats 030104 developmental biology embryonic structures Chloride channel glycine receptor Ion Channel Gating 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Bulletin of Experimental Biology and Medicine |
| ISSN: | 1573-8221 0007-4888 |
| DOI: | 10.1007/s10517-021-05125-3 |
| Popis: | Ivermectin (IVM) belongs to the class of macrocyclic lactones, which is used as an antiparasitic agent. At present, the researchers focus on possibility to use IVM in treatment of certain forms of cancer and viral diseases such as COVID-19. The mechanisms of IVM action are not clear. It is assumed that IVM affects chloride channels and increases cytoplasmic concentration of chloride. This study examines the effect of IVM on chloride currents induced by glycine (IGly). Experiments were carried out on isolated pyramidal neurons of the rat hippocampus with whole-cell patch clamp. A short-term (600 msec) application of IVM in a concentration of 10 μM induced a slow inward current, which persisted after washing the neurons. The low concentrations (0.1-1000 nM) of IVM did not induce any novel current, but it rapidly and reversibly reduced the peak amplitude and accelerated desensitization of IGly in a dose-dependent manner. The threshold concentrations of IVM sufficient to reduce peak amplitude of IGly and to accelerate desensitization of IGly were 100 nM and 0.1 nM, respectively. The study revealed a high sensitivity of neuronal glycine receptors to IVM. |
| Databáze: | OpenAIRE |
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