Larval Exposure to the Bacterial Insecticide Bti Enhances Dengue Virus Susceptibility of Adult Aedes aegypti Mosquitoes

Autor: Renaud Stalinski, Isabelle Moltini-Conclois, Louis Lambrechts, Laurence Després, Guillaume Tetreau
Přispěvatelé: Interactions Virus-Insectes - Insect-Virus Interactions (IVI), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Laboratoire d'Ecologie Alpine (LECA ), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), This work was supported by Agence Nationale de la Recherche (grant ANR-16-CE35-0004-01 to L.L.), the French Government’s Investissement d’Avenir program Laboratoire d’Excellence Integrative Biology of Emerging Infectious Diseases (grant ANR-10-LABX-62-IBEID to L.L.), the Région Rhône-Alpes (CIBLE program RA0000R464 to L.D.) and the City of Paris Emergence(s) program (to L.L.)., We thank Catherine Lallemand and Frédéric Laporte for assistance with mosquito rearing, Anna-Bella Failloux for sharing the CHIKV isolate, and three anonymous reviewers for constructive comments on an earlier version of the manuscript., ANR-16-CE35-0004,MOSQUIBIOTA,Contribution de la diversité bactérienne intestinale à la capacité vectorielle d'Aedes aegypti(2016), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
[SDV]Life Sciences [q-bio]
viruses
Human pathogen
Aedes aegypti
Dengue virus
medicine.disease_cause
Arbovirus
Virus
03 medical and health sciences
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
medicine
Chikungunya
Bacillus thuringiensis subsp. israelensis
lcsh:Science
Pathogen
Bacillus thuringiensis subsp israelensis
[SDV.GEN]Life Sciences [q-bio]/Genetics
vector competence
biology
Brief Report
fungi
virus diseases
multiple infection
medicine.disease
biology.organism_classification
Virology
3. Good health
030104 developmental biology
arbovirus
Insect Science
Vector (epidemiology)
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
lcsh:Q
carry-over effect
[SDV.EE.IEO]Life Sciences [q-bio]/Ecology
environment/Symbiosis
Zdroj: Insects, Vol 9, Iss 4, p 193 (2018)
Insects
Insects, MDPI, 2018, 9 (4), pp.193. ⟨10.3390/insects9040193⟩
Insects, 2018, 9 (4), pp.193. ⟨10.3390/insects9040193⟩
ISSN: 2075-4450
DOI: 10.3390/insects9040193⟩
Popis: Understanding the interactions between pathogens sharing the same host can be complicated for holometabolous animals when larval and adult stages are exposed to distinct pathogens. In medically important insect vectors, the effect of pathogen exposure at the larval stage may influence susceptibility to human pathogens at the adult stage. We addressed this hypothesis in the mosquito Aedes aegypti, a major vector of arthropod-borne viruses (arboviruses), such as the dengue virus (DENV) and the chikungunya virus (CHIKV). We experimentally assessed the consequences of sub-lethal exposure to the bacterial pathogen Bacillus thuringiensis subsp. israelensis (Bti), during larval development, on arbovirus susceptibility at the adult stage in three Ae. aegypti strains that differ in their genetic resistance to Bti. We found that larval exposure to Bti significantly increased DENV susceptibility, but not CHIKV susceptibility, in the Bti-resistant strains. However, there was no major difference in the baseline arbovirus susceptibility between the Bti-resistant strains and their Bti-susceptible parental strain. Although the generality of our results remains to be tested with additional arbovirus strains, this study supports the idea that the outcome of an infection by a pathogen depends on other pathogens sharing the same host even when they do not affect the same life stage of the host. Our findings may also have implications for Bti as a mosquito biocontrol agent, indicating that the sub-optimal Bti efficacy may have counter-productive effects by increasing vector competence, at least for some combinations of arbovirus and mosquito strains.
Databáze: OpenAIRE
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