Identification of candidate genes and mutations inQTL regions for immune responses in chicken
| Autor: | Tomasz Suchocki, Magdalena Fraszczak, K. Skiba, Joanna Szyda, Anna Slawinska, J. Skiba, Maria Siwek, Joanna Wesoly, Małgorzata Rydzanicz |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Lipopolysaccharides
Candidate gene Genotype Quantitative Trait Loci SNP chemical and pharmacologic phenomena Single-nucleotide polymorphism Adaptive Immunity Quantitative trait locus Biology Polymorphism Single Nucleotide Immune system avian genome Genetics Animals immune trait Models Statistical Innate immune system association Articles General Medicine Tag SNP Acquired immune system Immunity Innate Teichoic Acids Antibody Formation Hemocyanins Mutation Original Article Animal Science and Zoology quantitative trait nucleotide Chickens |
| Zdroj: | Animal Genetics |
| ISSN: | 1365-2052 0268-9146 |
| DOI: | 10.1111/age.12280 |
| Popis: | Summary There are two categories of immune responses – innate and adaptive immunity – both having polygenic backgrounds and a significant environmental component. In our study, adaptive immunity was represented by the specific antibody response toward keyhole limpet hemocyanin (KLH); innate immunity was represented by natural antibodies toward lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Defining genetic bases of immune responses leads from defining quantitative trait loci (QTL) toward a single mutation responsible for variation in the phenotypic trait. The goal of the reported study was to define candidate genes and mutations for the immune traits of interest in chicken by performing an association study of SNPs located in candidate genes defined in QTL regions. Candidate genes and SNPs in QTL regions were selected in silico. SNP association was based on a custom SNP panel, GoldenGate genotyping assay (Illumina) and two statistical models: random mixed model and CAR score. The most significant SNP for immune response toward KLH was located in the JMJD6 gene located on GGA18. Four SNPs in candidate genes FOXJ1 (GGA18), EPHB1 (GGA9), PTGER4 (GGAZ) and PRKCB (GGA14) showed association with natural antibodies for LPS. A single SNP in ITGB4 (GGA18) was associated with natural antibodies for LTA. All associated SNPs mentioned above showed additive effects. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |