Oxidative stress in cancer prone xeroderma pigmentosum fibroblasts. Real-time and single cell monitoring of superoxide and nitric oxide production with microelectrodes
| Autor: | Alain Sarasin, Neso Sojic, Delphine Bruce, Monique Vuillaume, Stéphane Arbault, Christian Amatore |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Xeroderma pigmentosum DNA Repair DNA repair Oxidative phosphorylation Nitric Oxide medicine.disease_cause Nitric oxide chemistry.chemical_compound Reference Values Superoxides Neoplasms Electrochemistry medicine Animals Fibroblast Cells Cultured Respiratory Burst Genetics Xeroderma Pigmentosum biology Superoxide Hydrogen Peroxide General Medicine Fibroblasts medicine.disease Molecular biology Oxidative Stress medicine.anatomical_structure chemistry Catalase biology.protein Microelectrodes Oxidative stress |
| Zdroj: | Carcinogenesis. 25:509-515 |
| ISSN: | 1460-2180 |
| DOI: | 10.1093/carcin/bgh046 |
| Popis: | Sun exposure is clearly implicated in premature skin ageing and neoplastic development. These features are exacerbated in patients with Xeroderma pigmentosum (XP), a hereditary disease associated at the cellular level with DNA repair defects and a low catalase activity. The implications of oxidative stress in the defects and cancer proneness of XP skin cells (keratinocytes, fibroblasts) are multiple and remain unclear. They were investigated here at the level of a single fibroblast by an electrochemical method based on microelectrodes we have developed previously. These microelectrodes permit a real-time quantification and identification of superoxide and nitric oxide derivatives (H 2 O 2 , ONOO-, NO○, NO - 2) released by a living cell following its stimulation. Then, the oxidative bursts produced by fibroblasts from normal strains were compared with those of fibroblasts from several XP group A (XPA) and XP group D (XPD) strains. All XPA and XPD strains provided responses of higher amplitude and duration than controls. The XP specific oxidative response could not be correlated with DNA repair ability since the transduction of XPD strains with the wild-type XPD gene did not modify their production of reactive oxygen and nitrogen species. The nature of these species was investigated and revealed that cancer prone XPD fibroblasts produced higher amounts of O○ - 2 and H 2 O 2 and lower amounts of NO○ and ONOO than normal fibroblasts. |
| Databáze: | OpenAIRE |
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