Multivalent Polymer–Peptide Conjugates: A General Platform for Inhibiting Amyloid Beta Peptide Aggregation
| Autor: | Yang Song, Giuseppe Léonardo Licari, Jeffrey S. Moore, Qian Chen, Edwin G. Moore, Emad Tajkhorshid, Chad M. Rienstra, John W. Smith, Abigail J. Halmes, Xing Jiang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
chemistry.chemical_classification
Polymers and Plastics biology Molecular mass Chemistry Amyloid beta Ligand Organic Chemistry Peptoid Peptide 02 engineering and technology Protein aggregation 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Article 0104 chemical sciences Inorganic Chemistry chemistry.chemical_compound Dynamic light scattering Materials Chemistry biology.protein Biophysics 0210 nano-technology Conjugate |
| Zdroj: | ACS Macro Letters. 8:1365-1371 |
| ISSN: | 2161-1653 |
| DOI: | 10.1021/acsmacrolett.9b00559 |
| Popis: | Protein aggregation is implicated in multiple deposition diseases including Alzheimer’s Disease, which features the formation of toxic aggregates of amyloid beta (Aβ) peptides. Many inhibitors have been developed to impede or reverse Aβ aggregation. Multivalent inhibitors, however, have been largely overlooked despite the promise of high inhibition efficiency endowed by the multivalent nature of Aβ aggregates. In this work, we report the success of multivalent polymer-peptide conjugates (mPPCs) as a general class of inhibitors of the aggregation of Aβ(40). Significantly delayed onset of fibril formation was realized using mPPCs prepared from three peptide/peptoid ligands covering a range of polymer molecular weights (MWs) and ligand loadings. Dose dependence studies showed that the nature of the ligands is a key factor in mPPC inhibition potency. The negatively charged ligand LPFFD (LD) leads to more efficient mPPCs compared to the neutral ligands, and is most effective at 7% ligand loading across different MWs. Molecular dynamics simulations along with dynamic light scattering experiments suggest that mPPCs form globular structures in solution due to ligand-ligand interactions. Such interactions are key to the spatial proximity of ligands and thus to the multivalency effect of mPPC inhibition. Excess ligand-ligand interactions, however, reduce the accessibility of mPPC ligands to Aβ peptides, and impair the overall inhibition potency. |
| Databáze: | OpenAIRE |
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