Cross-Regulation between a Novel Two-Component Signal Transduction System for Catabolism of Toluene in Pseudomonas mendocina and the TodST System from Pseudomonas putida
| Autor: | Maximino Manzanera, María Isabel Ramos-González, Gilberto Mosqueda, Anthony A. Gatenby, Susana Víchez, Juan L. Ramos, Monica Marie Olson, María Jesús Campos |
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| Rok vydání: | 2002 |
| Předmět: |
Genetics
Transcription Genetic Pseudomonas putida Operon Mutant Biology biology.organism_classification Microbiology Complementation Open reading frame Bacterial Proteins Multigene Family Pseudomonas Gene cluster Trans-Activators Gene Regulation Promoter Regions Genetic Protein Kinases Molecular Biology Gene Pseudomonas mendocina Signal Transduction Toluene |
| Zdroj: | Journal of Bacteriology. 184:7062-7067 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.184.24.7062-7067.2002 |
| Popis: | The tmoABCDEF genes encode the toluene-4-monooxygenase from Pseudomonas mendocina KR1. Upstream from the tmoA gene an open reading frame, tmoX , encoding a protein 83% identical to TodX ( todX being the initial gene in the todXFC1C2BADEGIH operon from Pseudomonas putida DOT-T1E) was found. The tmoX gene is also the initial gene in the tmoXABCDEF gene cluster. The transcription initiation point from the tmoX promoter was mapped, and the sequence upstream revealed striking identity with the promoter of the tod operon of P. putida . The tod operon is regulated by a two-component signal transduction system encoded by the todST genes. Two novel genes from P. mendocina KR1, tmoST , were rescued by complementation of a P. putida DOT-T1E todST knockout mutant, whose gene products shared about 85% identity with TodS-TodT. We show that transcription from P tmoX and P todX can be mediated by TmoS-TmoT or TodS-TodT, in the presence of toluene, revealing cross-regulation between these two catabolic pathways. |
| Databáze: | OpenAIRE |
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