Comparison of DNA ploidy status and DNA ploidy-related parameters in malignant melanoma tissue microarrays and full sections
| Autor: | Nadine Buschmann, Jerzy Stachura, Ulrich Brinck, G. Fischer, Carlos Cordon-Cardo, Monika Korabiowska |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
CAS200
Adult Male Pathology medicine.medical_specialty Skin Neoplasms Protein Array Analysis Feulgen stain Biology Pathology and Forensic Medicine 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine ploidy status melanoma Rosaniline Dyes medicine Humans Melanoma Aged Image Cytometry 030304 developmental biology Aged 80 and over 0303 health sciences Ploidies Tissue microarray tissue array Anatomical pathology DNA Neoplasm Middle Aged medicine.disease Tumor Pathology chemistry 030220 oncology & carcinogenesis Female DNA microarray Ploidy DNA |
| Zdroj: | Human Pathology. 35:887-891 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/j.humpath.2004.03.015 |
| Popis: | A new high-throughput tissue-arraying technique, now frequently used in tumor pathology, requires standardization of methods of DNA analysis, previously applied in full histological sections. The main objectives of this study were to evaluate DNA ploidy status and DNA ploidy-related parameters using the CAS200 image analyzer in malignant melanoma tissue microarrays and to compare them with full histological sections. Comparison of DNA ploidy-related parameters, including percentage of diploid cells, percentage of aneuploid cells between 2c and 4c, percentage of tetraploid cells, percentage of aneuploid cells between 4c and 8c, percentage of octaploid cells, percentage of 16-ploid cells, and 5c exceeding rate, did not reveal any significant differences between malignant melanoma tissue microarrays and full sections. The DNA ploidy status according to Auer differed in 1 out of 59 cases investigated. Our study demonstrated that it is possible to evaluate DNA ploidy status and DNA ploidy-related parameters in tissue microarrays, which is of practical relevance to tumor pathology. |
| Databáze: | OpenAIRE |
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