Short-chain fatty acids production and microbial community in sludge alkaline fermentation: Long-term effect of temperature
| Autor: | Baikun Li, Liu Ye, Bo Wang, Yue Yuan, Shuying Wang, Yongzhen Peng |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Acidogenesis
Environmental Engineering medicine.medical_treatment 0208 environmental biotechnology Carbohydrates Bioengineering 02 engineering and technology Alkalies 010501 environmental sciences Biology 01 natural sciences Hydrolysis Alkaliflexus Acetic acid chemistry.chemical_compound Bioreactors Bacterial Proteins medicine Waste Management and Disposal Phylogeny 0105 earth and related environmental sciences Waste Products Protease Bacteria Sewage Renewable Energy Sustainability and the Environment Fatty Acids Temperature alpha-Glucosidases Biodiversity General Medicine Fatty Acids Volatile biology.organism_classification 020801 environmental engineering Solubility chemistry Biochemistry Fermentation Alcaligenes |
| Zdroj: | Bioresource Technology. 211:685-690 |
| ISSN: | 0960-8524 |
| DOI: | 10.1016/j.biortech.2016.03.138 |
| Popis: | Sludge alkaline fermentation has been reported to achieve efficient short-chain fatty acids (SCFAs) production. Temperature played important role in further improved SCFAs production. Long-term SCFAs production from sludge alkaline fermentation was compared between mesotherm (30±2°C) and microtherm (15±2°C). The study of 90days showed that mesotherm led to 2.2-folds production of SCFAs as microtherm and enhanced the production of acetic acid as major component of SCFAs. Soluble protein and carbohydrate at mesotherm was 2.63-folds as that at microtherm due to higher activities of protease and α-glucosidase, guaranteeing efficient substrates to produce SCFAs. Illumina MiSeq sequencing revealed that microtherm increased the abundance of Corynebacterium, Alkaliflexus, Pseudomonas and Guggenheimella, capable of enhancing hydrolysis. Hydrolytic bacteria, i.e. Alcaligenes, Anaerolinea and Ottowia, were enriched at mesotherm. Meanwhile, acidogenic bacteria showed higher abundance at mesotherm than microtherm. Therefore, enrichment of functional bacteria and higher microbial activities resulted in the improved SCFAs at mesotherm. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect