Actively transcribed GAL genes can be physically linked to the nuclear pore by the SAGA chromatin modifying complex
| Autor: | Shana C. Kerr, Milo B. Fasken, Roopa Luthra, Luciano H. Apponi, Shyam S. Chaurasia, Suneela Ramineni, Sandro Roberto Valentini, Michelle T. Harreman, Anita H. Corbett |
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| Rok vydání: | 2006 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Histone acetyltransferase complex Transcription Genetic Recombinant Fusion Proteins Genes Fungal RNA-binding protein Saccharomyces cerevisiae Biology Biochemistry Nuclear pore Nuclear protein Molecular Biology Tandem affinity purification Genetics Binding Sites Nuclear Proteins RNA-Binding Proteins Cell Biology Chromatin Cell biology SAGA complex Kinetics Nuclear Pore Trans-Activators Chromatin immunoprecipitation Plasmids |
| Zdroj: | The Journal of biological chemistry. 282(5) |
| ISSN: | 0021-9258 |
| Popis: | Recent work has demonstrated that some actively transcribed genes closely associate with nuclear pore complexes (NPC) at the nuclear periphery. The Saccharomyces cerevisiae Mlp1 and Mlp2 proteins are components of the inner nuclear basket of the nuclear pore that mediate interactions with these active genes. To investigate the physical link between the NPC and active loci, we identified proteins that interact with the carboxyl-terminal globular domain of Mlp1 by tandem affinity purification coupled with mass spectrometry. This analysis led to the identification of several components of the Spt-Ada-Gcn5-acetyltransferase (SAGA) histone acetyltransferase complex, Gcn5, Ada2, and Spt7. We utilized co-immunoprecipitation and in vitro binding assays to confirm the interaction between the Mlp proteins and SAGA components. Chromatin immunoprecipitation experiments revealed that Mlp1 and SAGA components associate with the same region of the GAL promoters. Critically, this Mlp-promoter interaction depends on the integrity of the SAGA complex. These results identify a physical association between SAGA and the NPC, and support previous results that relied upon visualization of GAL loci at the nuclear periphery by microscopy (Cabal, G. G. Genovesio, A., Rodriguez-Navarro, S., Zimmer, C., Gadal, O., Lesne, A., Buc, H., Feuerbach-Fournier, F., Olivo-Marin, J.-C., Hurt, E. C., and Nehrbass, U. (2006) Nature 441, 770-773). We propose that a physical interaction between nuclear pore components and the SAGA complex can link the actively transcribed GAL genes to the nuclear pore. |
| Databáze: | OpenAIRE |
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