Photocontrollable Probes for Mitochondrial Protein Profiling

Autor:	Shuhong Guo, Chaonan Yuan, Wenjie Lang, Danqi Hong, Jian Liu, Jintao Huang, Jia Dong, Jingyan Ge
Rok vydání:	2022
Předmět:	Mitochondrial Proteins Proteomics Proteome Organic Chemistry Humans Molecular Medicine Molecular Biology Biochemistry HeLa Cells Mitochondria
Zdroj:	ChemBioChem. 23
ISSN:	1439-7633 1439-4227
DOI:	10.1002/cbic.202200066
Popis:	The mitochondrion is the core site of cell signaling, energy metabolism and biosynthesis. Here, taking advantage of activity-based probes, we synthesized two photocontrollable probes (YGH-1 and YGH-2), composed of a mitochondrial localization moiety "triphenylphosphonium", a photo-triggered group to achieve spatially and temporally controlled protein capture, and an alkyne group to enrich the labeled protein. Proteomic validation was further carried out to facilitate identification of the mitochondrial proteome in HeLa cells. The results show that half of the identified protein hits (∼300) labeled by YGH-1 and YGH-2 belong to mitochondria, and are mostly localized in the mitochondrial matrix and inner mitochondrial membrane. Our results provide a new tool for spatial and temporal analysis of subcellular proteomes.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::b7b06ecc8a2b6c748a8fa2b372f72372 https://doi.org/10.1002/cbic.202200066 Zobrazit plný text záznamu Plný text