Monitoring and traceability of genetically modified soya bean event GTS 40-3-2 during soya bean protein concentrate and isolate preparation
| Autor: | Fusheng Chen, Yan Du, Chen Chen, Kunlun Liu |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Soya bean
soya bean protein concentrate Raw material Biology soya bean protein isolate 01 natural sciences 0404 agricultural biotechnology DNA degradation Labelling Food science lcsh:Science Multidisciplinary 010401 analytical chemistry food and beverages Lectin 04 agricultural and veterinary sciences 040401 food science 0104 chemical sciences Genetically modified organism Chemistry traceability biology.protein DNA fragmentation lcsh:Q genetically modified soya bean real-time PCR Research Article |
| Zdroj: | Royal Society Open Science Royal Society Open Science, Vol 7, Iss 10 (2020) |
| ISSN: | 2054-5703 |
| DOI: | 10.1098/rsos.201147 |
| Popis: | To evaluate DNA fragmentation and GMO quantification during soya bean protein concentrate and isolate preparation, genetically modified soya bean event GTS 40-3-2 (Roundup Ready TM soya bean, RRS) was blended with conventional soya beans at mass percentages of 0.9%, 2%, 3%, 5% and 10%. Qualitative PCR and real-time PCR were used to monitor the taxon-specific lectin and exogenous cp4 epsps target levels in all of the main products and by-products, which has practical significance for RRS labelling threshold and traceability. Along the preparation chain, the majority of DNA was distributed in main products, and the DNA degradation was noticed. From a holistic perspective, the lectin target degraded more than cp4 epsps target during both of the two soya bean proteins preparations. Therefore, the transgenic contents in the final protein products were higher than the actual mass percentages of RRS in raw materials. Our results are beneficial to the improvement of GMO labelling legislation and the protection of consumer rights. |
| Databáze: | OpenAIRE |
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