Simultaneous quantification of idelalisib, fludarabine and lenalidomide in rat plasma by using high-performance liquid chromatography coupled with heated electrospray ionization tandem mass spectrometry
| Autor: | Srikant Viswanadha, Santhoshkumar Nalla, Manikantakumar Golla, Sridhar Veeraraghavan, Manivannan Rangasamy, Swaroopkumar Vakkalanka, Satheeshmanikandan R S Thappali, Sandhyarani Chennupati |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
Bioanalysis Spectrometry Mass Electrospray Ionization Formic acid Electrospray ionization Clinical Biochemistry Analytical chemistry Mass spectrometry Tandem mass spectrometry Biochemistry High-performance liquid chromatography Sensitivity and Specificity Analytical Chemistry chemistry.chemical_compound Drug Stability Tandem Mass Spectrometry medicine Animals Rats Wistar Lenalidomide Chromatography High Pressure Liquid Quinazolinones Chromatography Chemistry Reproducibility of Results Cell Biology General Medicine Fludarabine Rats Thalidomide Purines Idelalisib Vidarabine medicine.drug |
| Zdroj: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. |
| ISSN: | 1873-376X |
| Popis: | A sensitive and reliable high-performance liquid chromatography-mass spectrometry (LC-MS/MS) method was developed and validated for simultaneous quantification of idelalisib, fludarabine and lenalidomide using tolbutamide as an internal standard. Analytes were recovered by liquid-liquid extraction and separated on a reverse phase C18 column (150mm×4.6mm i.d., 5μm) using methanol:0.1% formic acid buffer (70:30) as mobile phase at a flow rate of 1mL/min in isocratic mode. Selective reaction monitoring was performed using the transitions, i.e. m/z 416.25/176.48, 286.11/154.10, 260.15/149.15, and 271.14/155.06 to quantify idelalisib, fludarabine and lenalidomide and tolbutamide, respectively. The method was validated over the concentration range of 1.15-576.84ng/mL for idelalisib, 0.95-476.25ng/mL for fludarabine and 0.97-486.19ng/mL for lenalidomide. Intra and inter-day accuracy and precision of validated method were within the acceptable limits of15%. Coefficients of correlation (r(2)) for the calibration curves were0.998 for all analytes. The method was successfully applied for simultaneous estimation of idelalisib, fludarabine and lenalidomide in a pharmacokinetic study in rats. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect