The bi-directional transcriptional promoters for the latency-relating transcripts of the pp38/pp24 mRNAs and the 1.8 kb-mRNA in the long inverted repeats of Marek’s disease virus serotype 1 DNA are regulated by common promoter-specific enhancers
| Autor: | Kanji Hirai, Masashi Sakaguchi, Yasushi Kawaguchi, M. Shirakata, H. Shigekane |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Chloramphenicol O-Acetyltransferase
Gene Expression Regulation Viral Transcription Genetic Inverted repeat Molecular Sequence Data Transfection Cell Line Viral Proteins Genes Reporter Transcription (biology) Virology Virus latency medicine RNA Messenger Promoter Regions Genetic Enhancer Antigens Viral Herpesvirus 2 Gallid Gene Repetitive Sequences Nucleic Acid Reporter gene Marek's disease Base Sequence biology Promoter General Medicine Phosphoproteins biology.organism_classification medicine.disease Molecular biology Virus Latency Enhancer Elements Genetic DNA Viral Plasmids |
| Zdroj: | Archives of Virology. 144:1893-1907 |
| ISSN: | 1432-8798 0304-8608 |
| DOI: | 10.1007/s007050050713 |
| Popis: | In cell lines established from Marek's disease tumors, several viral transcripts are expressed and among them the products of pp38/pp24 mRNA and 1.8 kb-mRNA have been suggested to be involved in viral oncogenicity. The long inverted repeats of Marek's Disease virus serotype 1 (MDV1) genome contain closely located transcriptional promoters for phosphorylated protein pp38/pp24 and 1.8 kb-mRNA. These promoters initiate transcription in opposite directions and are separated only by a short enhancer region, which is likely to regulate both promoters simultaneously. We have analyzed the transcription activity of these promoters in MDV1 (Md5 strain) infected CEF by transient expression of CAT reporter genes and found that the promoters were in fact active in infected cells and the promoter for 1.8 kb-mRNA was more active than the pp38/pp24 promoter. Deletion analysis of the short enhancer region revealed that the 30 bp region overlapping the enhancer elements for 1.8 kb-mRNA was important for promoter activity for pp38/pp24. The gel shift analysis revealed that nuclear factor(s) actually bound to the overlapping 30 bp region. In addition, the activity of these promoters in infected cells varied with MDV strains. These results suggest that pp38/pp24 and 1.8 kb-mRNA promoters share a common regulatory sequence but a viral or a cellular factor(s) induced by viral infection regulates the promoter by distinct mechanisms. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink