The effect of anti-tuberculosis drugs therapy on mRNA efflux pump gene expression of Rv1250 in Mycobacterium tuberculosis collected from tuberculosis patients
| Autor: | Faiqah Umar, Rosdiana Natzir, Mochammad Hatta, Ressy Dwiyanti, Dirayah Rauf Husain, Muhammad Reza Primaguna, Ade Rifka Junita |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Tuberculosis biology business.industry 030106 microbiology Drug resistance medicine.disease biology.organism_classification Microbiology lcsh:Infectious and parasitic diseases Mycobacterium tuberculosis 03 medical and health sciences 030104 developmental biology Infectious Diseases Real-time polymerase chain reaction Gene expression Medicine lcsh:RC109-216 RNA extraction Efflux business Gene |
| Zdroj: | New Microbes and New Infections, Vol 32, Iss, Pp-(2019) |
| ISSN: | 2052-2975 |
| Popis: | Efflux pumps are transmembrane proteins that vigorously participate in extruding a wide range of substrates, including drugs, outside the bacterial cell. We aimed to investigate the mRNA expression level of the Rv1250 efflux pump gene in Mycobacterium tuberculosis isolated from individuals with tuberculosis who received drug therapy, at the 1st, 3rd and 5th months, and newly diagnosed patients with tuberculosis who will receive drug therapy (0 month). The study was a multiple cross-sectional longitudinal design—50 different M. tuberculosis isolates and a reference strain H37Rv were subcultured in LJ medium and confirmed by multiplex PCR for identification of M. tuberculosis and collected for RNA extraction. Total bacterial mRNA was analysed using real-time quantitative PCR to evaluate mRNA quantification gene expression. There were differences in the level of Rv1250 mRNA expression between sensitive (n = 11) and resistant (n = 40) groups of 5.961 ± 0.414 and 10.192 ± 1.978, respectively (fold changes; p |
| Databáze: | OpenAIRE |
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