Overexpression of calcium-binding protein calgranulin B in colonic mucosal diseases
| Autor: | František Langr, Jiří Stulík, Aleš Macela, Albrecht Otto, Bernd Thiede, Bures J, Jandík P, Hana Kovářová, Peter R. Jungblut |
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| Rok vydání: | 1997 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Colorectal cancer Clinical Biochemistry Colonic Polyps Inflammation Biology Peptide Mapping Biochemistry Inflammatory bowel disease Mass Spectrometry Colonic Diseases Calcium-binding protein medicine Calgranulin B Humans Calgranulin Electrophoresis Gel Two-Dimensional Amino Acid Sequence Intestinal Mucosa Aged Gel electrophoresis Two-dimensional gel electrophoresis Calcium-Binding Proteins Biochemistry (medical) Reproducibility of Results General Medicine Hydrogen-Ion Concentration Middle Aged Inflammatory Bowel Diseases medicine.disease digestive system diseases biology.protein Female medicine.symptom Colorectal Neoplasms |
| Zdroj: | Clinica Chimica Acta. 265:41-55 |
| ISSN: | 0009-8981 |
| DOI: | 10.1016/s0009-8981(97)00101-0 |
| Popis: | Subtractive two-dimensional gel electrophoresis (2-DE) has been used for the study of the protein patterns of the normal colonic mucosa and the specimens collected from patients diagnosed for inflammatory bowel disease (IBD), colonic polyps and colorectal cancer. We found a 13 kDa protein that was detected in five of seven adenomas and in 13 of 15 colorectal carcinomas while it was absent or only slightly expressed in normal colonic mucosa. Furthermore, this protein occured in all specimens collected from patients suffering from IBD and its quantity reflected the increased severity of inflammation. The combination of microsequencing and mass spectrometry led to the identification of the 13 kDa spot as calgranulin B. Our results indicate that the production of calgranulin B is unregulated in inflammatory, preneoplastic and neoplastic lesions of colonic mucosa. |
| Databáze: | OpenAIRE |
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