Potential use of bacterial community succession for estimating post-mortem interval as revealed by high-throughput sequencing
| Autor: | Zhenyu Hu, Weitao Yan, Jie Yan, Yunfeng Chang, Jifeng Cai, Lagabaiyila Zha, Juanjuan Guo, Yanjun Ding, Huidan Liao, Yadong Guo, Lingling Long, Xiaoliang Fu |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
DNA
Bacterial 0301 basic medicine Time Factors Firmicutes Zoology Polymerase Chain Reaction Article Rats Sprague-Dawley 03 medical and health sciences 0302 clinical medicine RNA Ribosomal 16S Proteobacteria Botany Gammaproteobacteria Animals 030216 legal & forensic medicine Relative species abundance Post-mortem interval Mouth Multidisciplinary Bacteria biology Bacteroidetes Phylum Microbiota Mouth Mucosa Rectum High-Throughput Nucleotide Sequencing biology.organism_classification 030104 developmental biology Microbial population biology Female Autopsy |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/srep24197 |
| Popis: | Decomposition is a complex process involving the interaction of both biotic and abiotic factors. Microbes play a critical role in the process of carrion decomposition. In this study, we analysed bacterial communities from live rats and rat remains decomposed under natural conditions, or excluding sarcosaphagous insect interference, in China using Illumina MiSeq sequencing of 16S rRNA gene amplicons. A total of 1,394,842 high-quality sequences and 1,938 singleton operational taxonomic units were obtained. Bacterial communities showed notable variation in relative abundance and became more similar to each other across body sites during the decomposition process. As decomposition progressed, Proteobacteria (mostly Gammaproteobacteria) became the predominant phylum in both the buccal cavity and rectum, while Firmicutes and Bacteroidetes in the mouth and rectum, respectively, gradually decreased. In particular, the arrival and oviposition of sarcosaphagous insects had no obvious influence on bacterial taxa composition, but accelerated the loss of biomass. In contrast to the rectum, the microbial community structure in the buccal cavity of live rats differed considerably from that of rats immediately after death. Although this research indicates that bacterial communities can be used as a “microbial clock” for the estimation of post-mortem interval, further work is required to better understand this concept. |
| Databáze: | OpenAIRE |
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