The relationship between gut microbiota and short chain fatty acids in the renal calcium oxalate stones disease
| Autor: | Zude Chen, Zhongyu Jian, Hyokyoung Grace Hong, Liyuan Xiang, Qun Sun, Yucheng Ma, Shiqian Qi, Zhitao Wei, Hong Li, Yi Li, Jianzhong Ai, Qingyao Jiang, Yu Liu, Liang Cheng, Xi Jin, Kunjie Wang |
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| Rok vydání: | 2020 |
| Předmět: |
Male
0301 basic medicine medicine.medical_specialty medicine.drug_class Urinary system Antibiotics Calcium oxalate Gut flora Kidney Nephrolithiasis Biochemistry Oxalate Rats Sprague-Dawley Feces Kidney Calculi 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine RNA Ribosomal 16S Internal medicine Genetics medicine Animals Humans Molecular Biology Bacteria Calcium Oxalate biology Middle Aged Fatty Acids Volatile biology.organism_classification medicine.disease Gastrointestinal Microbiome Rats 030104 developmental biology Endocrinology chemistry Case-Control Studies Kidney stones Metagenomics 030217 neurology & neurosurgery Biotechnology |
| Zdroj: | The FASEB Journal. 34:11200-11214 |
| ISSN: | 1530-6860 0892-6638 |
| DOI: | 10.1096/fj.202000786r |
| Popis: | The relationship of gut microbiota and calcium oxalate stone has been limited investigated, especially with no study of gut microbiota and short chain fatty acids (SCFAs) in nephrolithiasis. We provided Sprague Dawley rats of renal calcium oxalate stones with antibiotics and examined the renal crystals deposition. We also performed a case-control study by analyzing 16S rRNA microbial profiling, shotgun metagenomics and SCFAs in 153 fecal samples from non-kidney stone (NS) controls, patients with occasional renal calcium oxalate stones (OS) and patients with recurrent stones (RS). Antibiotics reduced bacterial load in feces and could promote the formation of renal calcium crystals in model rats. In addition, both OS and RS patients exhibited higher fecal microbial diversity than NS controls. Several SCFAs-producing gut bacteria, as well as metabolic pathways associated with SCFAs production, were considerably lower in the gut microbiota among the kidney stone patients compared with the NS controls. Representation of genes involved in oxalate degradation showed no significance difference among groups. However, fecal acetic acid concentration was the highest in RS patients with high level of urinary oxalate, which was positively correlated with genes involvement in oxalate synthesis. Administration of SCFAs reduced renal crystals. These results shed new light on bacteria and SCFAs, which may promote the development of treatment strategy in nephrolithiasis. |
| Databáze: | OpenAIRE |
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