Imaging and SERS Study of the Au Nanoparticles Interaction with HPV and Carcinogenic Cervical Tissues

Autor: Tzarara López-Luke, Juan L. Vivero-Escoto, Ramón Carriles, Ana Lilia Gonzalez-Yebra, Andrea Ceja-Fdez, Elder De la Rosa
Rok vydání: 2021
Předmět:
inorganic chemicals
HPV
cervical cancer
Confocal
Metal Nanoparticles
Uterine Cervical Neoplasms
Pharmaceutical Science
Infrared spectroscopy
Nanoparticle
Spectrum Analysis
Raman
confocal microscopy
01 natural sciences
Article
Analytical Chemistry
law.invention
010309 optics
symbols.namesake
QD241-441
Confocal microscopy
law
0103 physical sciences
Drug Discovery
Humans
Physical and Theoretical Chemistry
Spectroscopy
Papillomaviridae
Chemistry
Papillomavirus Infections
010401 analytical chemistry
Organic Chemistry
Raman spectroscopy and SERS
technology
industry
and agriculture
DNA
Surface-enhanced Raman spectroscopy
0104 chemical sciences
Chemistry (miscellaneous)
Colloidal gold
gold nanoparticles
multiphoton microscopy
Microscopy
Electron
Scanning
Biophysics
symbols
Molecular Medicine
Female
Gold
two-photon imaging
Raman spectroscopy
Zdroj: Molecules
Volume 26
Issue 12
Molecules, Vol 26, Iss 3758, p 3758 (2021)
ISSN: 1420-3049
DOI: 10.3390/molecules26123758
Popis: In this work, gold NPs were prepared by the Turkevich method, and their interaction with HPV and cancerous cervical tissues were studied by scanning electron microscopy, energy-dispersive x-ray spectroscopy, confocal and multiphoton microscopy and SERS. The SEM images confirmed the presence and localization of the gold NPs inside of the two kinds of tissues. The light absorption of the gold NPs was at 520 nm. However, it was possible to obtain two-photon imaging (red emission region) of the gold NPs inside of the tissue, exciting the samples at 900 nm, observing the morphology of the tissues. The infrared absorption was probably due to the aggregation of gold NPs inside the tissues. Therefore, through the interaction of gold nanoparticles with the HPV and cancerous cervical tissues, a surface enhanced Raman spectroscopy (SERS) was obtained. As preliminary studies, having an average of 1000 Raman spectra per tissue, SERS signals showed changes between the HPV-infected and the carcinogenic tissues
these spectral signatures occurred mainly in the DNA bands, potentially offering a tool for the rapid screening of cancer.
Databáze: OpenAIRE
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